Comparison of Common Enrichment Broths Used in Diagnostic Laboratories for Shiga Toxin-Producing

Overview

Journal Microorganisms

Specialty Microbiology

Date 2021 Mar 6

PMID 33673617

Citations 3

Authors

Michael Bording-Jorgensen

Hannah Tyrrell

Colin Lloyd

Linda Chui

Affiliations

Soon will be listed here.

Abstract

Acute gastroenteritis caused by Shiga toxin-producing (STEC) affects more than 4 million individuals in Canada. Diagnostic laboratories are shifting towards culture-independent diagnostic testing; however, recovery of STEC remains an important aspect of surveillance programs. The objective of this study was to compare common broth media used for the enrichment of STEC. Clinical isolates including O157:H7 as well as non-O157 serotypes were cultured in tryptic soy (TSB), MacConkey (Mac), and Gram-negative (GN) broths and growth was compared using culture on sheep's blood agar and real-time PCR (qPCR). In addition, a selection of the same isolates was spiked into negative stool and enriched in the same three broths, which were then evaluated using culture on CHROMagar STEC agar and qPCR. TSB was found to provide the optimal enrichment for growth of isolates with and without stool. The results from this study suggest that diagnostic laboratories may benefit from enriching STEC samples in TSB as a first line enrichment instead of GN or Mac.

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