Candidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative Lectin/Immunohistochemical Assays

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2021 Mar 6

PMID 33673507

Citations 1

Authors

Patharee Oungsakul

Eunju Choi

Alok K Shah

Ahmed Mohamed

Caroline OLeary

David Duffy

Michelle M Hill

Helle Bielefeldt-Ohmann

Affiliations

Soon will be listed here.

Abstract

Visceral hemangiosarcoma (HSA) is one of the more frequent cancers in dogs and has a high metastatic rate and poor prognosis, as clinical signs only become apparent in advanced stages of tumor development. In order to improve early and differential diagnostic capabilities and hence, prognosis for dogs with HSA, two types of biomarker are needed: a point-of-care diagnostic biomarker and a prognostic biomarker-preferentially based on samples obtained with minimally invasive methods. In this study, we applied a lectin magnetic bead array-coupled tandem mass spectrometry (LeMBA-MS/MS) workflow through discovery and validation phases to discover serum glycoprotein biomarker candidates for canine HSA. By this approach, we found that (DSA), wheat germ agglutinin (WGA), (SNA), and (PSA) lectins captured the highest number of validated candidate glycoproteins. Secondly, we independently validated serum LeMBA-MS/MS results by demonstrating the in situ relationship of lectin-binding with tumor cells. Using lectin-histochemistry and immunohistochemistry (IHC) for key proteins on tissues with HSA and semi-quantitation of the signals, we demonstrate that a combination of DSA histochemistry and IHC for complement C7 greatly increases the prospect of a more specific diagnosis of canine HSA.

Citing Articles

Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer.

Dutt M, Hartel G, Richards R, Shah A, Mohamed A, Apostolidou S Proteomics Clin Appl. 2023; 17(4):e2200114.

PMID: 37147936 PMC: 7615076. DOI: 10.1002/prca.202200114.

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