Biotechnological Production of the Sunscreen Pigment Scytonemin in Cyanobacteria: Progress and Strategy

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2021 Mar 6

PMID 33673485

Citations 11

Authors

Xiang Gao

Xin Jing

Xufeng Liu

Peter Lindblad

Affiliations

Soon will be listed here.

Abstract

Scytonemin is a promising UV-screen and antioxidant small molecule with commercial value in cosmetics and medicine. It is solely biosynthesized in some cyanobacteria. Recently, its biosynthesis mechanism has been elucidated in the model cyanobacterium PCC 73102. The direct precursors for scytonemin biosynthesis are tryptophan and -hydroxyphenylpyruvate, which are generated through the shikimate and aromatic amino acid biosynthesis pathway. More upstream substrates are the central carbon metabolism intermediates phosphoenolpyruvate and erythrose-4-phosphate. Thus, it is a long route to synthesize scytonemin from the fixed atmospheric CO in cyanobacteria. Metabolic engineering has risen as an important biotechnological means for achieving sustainable high-efficiency and high-yield target metabolites. In this review, we summarized the biochemical properties of this molecule, its biosynthetic gene clusters and transcriptional regulations, the associated carbon flux-driving progresses, and the host selection and biosynthetic strategies, with the aim to expand our understanding on engineering suitable cyanobacteria for cost-effective production of scytonemin in future practices.

Citing Articles

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References

Itoh T, Tsuzuki R, Tanaka T, Ninomiya M, Yamaguchi Y, Takenaka H . Reduced scytonemin isolated from Nostoc commune induces autophagic cell death in human T-lymphoid cell line Jurkat cells. Food Chem Toxicol. 2013; 60:76-82. DOI: 10.1016/j.fct.2013.07.016. View

Miao R, Xie H, Liu X, Lindberg P, Lindblad P . Current processes and future challenges of photoautotrophic production of acetyl-CoA-derived solar fuels and chemicals in cyanobacteria. Curr Opin Chem Biol. 2020; 59:69-76. DOI: 10.1016/j.cbpa.2020.04.013. View

Rastogi R, Sonani R, Madamwar D . Cyanobacterial Sunscreen Scytonemin: Role in Photoprotection and Biomedical Research. Appl Biochem Biotechnol. 2015; 176(6):1551-63. DOI: 10.1007/s12010-015-1676-1. View

Kang M, Jo S, Lee H, Yoon Y, Kwon J, Yang J . Inhibition of Skin Inflammation by Scytonemin, an Ultraviolet Sunscreen Pigment. Mar Drugs. 2020; 18(6). PMC: 7344946. DOI: 10.3390/md18060300. View

Stevenson C, Capper E, Roshak A, Marquez B, Grace K, Gerwick W . Scytonemin--a marine natural product inhibitor of kinases key in hyperproliferative inflammatory diseases. Inflamm Res. 2002; 51(2):112-4. DOI: 10.1007/BF02684014. View

Strebhardt K, Ullrich A . Targeting polo-like kinase 1 for cancer therapy. Nat Rev Cancer. 2006; 6(4):321-30. DOI: 10.1038/nrc1841. View

Patra K, Hay N . The pentose phosphate pathway and cancer. Trends Biochem Sci. 2014; 39(8):347-54. PMC: 4329227. DOI: 10.1016/j.tibs.2014.06.005. View

Oldiges M, Kunze M, Degenring D, Sprenger G, Takors R . Stimulation, monitoring, and analysis of pathway dynamics by metabolic profiling in the aromatic amino acid pathway. Biotechnol Prog. 2004; 20(6):1623-33. DOI: 10.1021/bp0498746. View

Bongaerts J, Kramer M, Muller U, Raeven L, Wubbolts M . Metabolic engineering for microbial production of aromatic amino acids and derived compounds. Metab Eng. 2001; 3(4):289-300. DOI: 10.1006/mben.2001.0196. View

10.

Balskus E, Walsh C . Investigating the initial steps in the biosynthesis of cyanobacterial sunscreen scytonemin. J Am Chem Soc. 2008; 130(46):15260-1. PMC: 2631159. DOI: 10.1021/ja807192u. View

11.

Budel B, Karsten U, Garcia-Pichel F . Ultraviolet-absorbing scytonemin and mycosporine-like amino acid derivatives in exposed, rock-inhabiting cyanobacterial lichens. Oecologia. 2017; 112(2):165-172. DOI: 10.1007/s004420050296. View

12.

Hasunuma T, Matsuda M, Kato Y, Vavricka C, Kondo A . Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803. Metab Eng. 2018; 48:109-120. DOI: 10.1016/j.ymben.2018.05.013. View

13.

Brey L, Wlodarczyk A, Bang Thofner J, Burow M, Crocoll C, Nielsen I . Metabolic engineering of Synechocystis sp. PCC 6803 for the production of aromatic amino acids and derived phenylpropanoids. Metab Eng. 2019; 57:129-139. DOI: 10.1016/j.ymben.2019.11.002. View

14.

Balskus E, Case R, Walsh C . The biosynthesis of cyanobacterial sunscreen scytonemin in intertidal microbial mat communities. FEMS Microbiol Ecol. 2011; 77(2):322-32. PMC: 3134115. DOI: 10.1111/j.1574-6941.2011.01113.x. View

15.

Garcia-Pichel F, Lombard J, Soule T, Dunaj S, Wu S, Wojciechowski M . Timing the Evolutionary Advent of Cyanobacteria and the Later Great Oxidation Event Using Gene Phylogenies of a Sunscreen. mBio. 2019; 10(3). PMC: 6529634. DOI: 10.1128/mBio.00561-19. View

16.

Balskus E, Walsh C . An enzymatic cyclopentyl[b]indole formation involved in scytonemin biosynthesis. J Am Chem Soc. 2009; 131(41):14648-9. PMC: 2766776. DOI: 10.1021/ja906752u. View

17.

Garcia-Pichel F, Sherry N, Castenholz R . Evidence for an ultraviolet sunscreen role of the extracellular pigment scytonemin in the terrestrial cyanobacterium Chlorogloeopsis sp. Photochem Photobiol. 1992; 56(1):17-23. DOI: 10.1111/j.1751-1097.1992.tb09596.x. View

18.

Fordjour E, Adipah F, Zhou S, Du G, Zhou J . Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of L-DOPA from D-glucose. Microb Cell Fact. 2019; 18(1):74. PMC: 6482505. DOI: 10.1186/s12934-019-1122-0. View

19.

Liu Q, Cheng Y, Xie X, Xu Q, Chen N . Modification of tryptophan transport system and its impact on production of L-tryptophan in Escherichia coli. Bioresour Technol. 2012; 114:549-54. DOI: 10.1016/j.biortech.2012.02.088. View

20.

Cui L, Xu H, Zhu Z, Gao X . The effects of the exopolysaccharide and growth rate on the morphogenesis of the terrestrial filamentous cyanobacterium . Biol Open. 2017; 6(9):1329-1335. PMC: 5612240. DOI: 10.1242/bio.026955. View