Expression of Apoptosis-Related Genes in Cat Testicular Tissue in Relation to Sperm Morphology and Seasonality-A Preliminary Study

Overview

Journal Animals (Basel)

Date 2021 Mar 6

PMID 33673379

Citations 2

Authors

Sylwia Prochowska

Agnieszka Partyka

Wojciech Nizanski

Affiliations

Soon will be listed here.

Abstract

Apoptosis is a crucial process in spermatogenesis, responsible for the elimination of abnormal sperm cells and testicular regression out of breeding season. The aim of this study was to assess if the expression of apoptosis-related genes in testicular tissue of domestic cats differed: (1) between normozoospermic and teratozoospermic donors, and (2) between reproductive and non-reproductive season. The expression of genes: , , , and caspases (, and ) was analyzed by qRT-PCR in testicular tissue samples. During non-reproductive season significantly higher expression of two anti-apoptotic genes ( and ) was observed. Additionally, there was a significant higher expression of in teratozoospermic cats during non-reproductive than during reproductive season. No differences were noted between normozoospermic and teratozoospermic groups. Upregulation of some genes during the non-reproductive season indicates engagement of apoptotic mechanisms in the seasonal changes of semen quality in cats, however further studies on protein levels and analysis of changes on distinct testicular germinal layers are required. At the same time, teratozoospermia in the general population of cats seems to be not connected with dysregulation of apoptosis in the testes.

Citing Articles

Seasonal Influence on the Impact of Human Chorionic Gonadotropin (hCG) on Testicular Haemodynamics and Hormonal Responses in Canines: A Comparative Study.

Nadali B, Abdous A, Karami N, Jokar M, Amarloie O, Farhoodi M Vet Med Sci. 2024; 10(6):e70117.

PMID: 39535346 PMC: 11558201. DOI: 10.1002/vms3.70117.

Proliferation and Apoptosis of Cat () Male Germ Cells during Breeding and Non-Breeding Seasons.

Valentini L, Zupa R, Pousis C, Cuko R, Corriero A Vet Sci. 2022; 9(8).

PMID: 36006362 PMC: 9414637. DOI: 10.3390/vetsci9080447.

References

Tsutsui T, Onodera F, Oba H, Mizutani T, Hori T . Plasma hormone levels and semen quality in male cats during non-breeding and breeding seasons. Reprod Domest Anim. 2009; 44 Suppl 2:291-3. DOI: 10.1111/j.1439-0531.2009.01449.x. View

Neubauer K, Jewgenow K, Blottner S, Wildt D, Pukazhenthi B . Quantity rather than quality in teratospermic males: a histomorphometric and flow cytometric evaluation of spermatogenesis in the domestic cat (Felis catus). Biol Reprod. 2004; 71(5):1517-24. DOI: 10.1095/biolreprod.104.031062. View

Pukazhenthi B, Neubauer K, Jewgenow K, Howard J, Wildt D . The impact and potential etiology of teratospermia in the domestic cat and its wild relatives. Theriogenology. 2006; 66(1):112-21. DOI: 10.1016/j.theriogenology.2006.03.020. View

Lue Y, Hikim A, Swerdloff R, Im P, Taing K, Bui T . Single exposure to heat induces stage-specific germ cell apoptosis in rats: role of intratesticular testosterone on stage specificity. Endocrinology. 1999; 140(4):1709-17. DOI: 10.1210/endo.140.4.6629. View

Howard J, Brown J, Bush M, Wildt D . Teratospermic and normospermic domestic cats: ejaculate traits, pituitary-gonadal hormones, and improvement of spermatozoal motility and morphology after swim-up processing. J Androl. 1990; 11(3):204-15. View

Odorisio T, Rodriguez T, Evans E, Clarke A, Burgoyne P . The meiotic checkpoint monitoring synapsis eliminates spermatocytes via p53-independent apoptosis. Nat Genet. 1998; 18(3):257-61. DOI: 10.1038/ng0398-257. View

Blanco-Rodriguez J . A matter of death and life: the significance of germ cell death during spermatogenesis. Int J Androl. 1998; 21(5):236-48. DOI: 10.1046/j.1365-2605.1998.00133.x. View

Shinoda K, Mitsumori K, Yasuhara K, Uneyama C, Onodera H, Takegawa K . Involvement of apoptosis in the rat germ cell degeneration induced by nitrobenzene. Arch Toxicol. 1998; 72(5):296-302. DOI: 10.1007/s002040050505. View

Zambelli D, Prati F, Cunto M, Iacono E, Merlo B . Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration. Theriogenology. 2007; 69(4):485-90. DOI: 10.1016/j.theriogenology.2007.10.019. View

10.

Young K, Nelson R . Mediation of seasonal testicular regression by apoptosis. Reproduction. 2001; 122(5):677-85. DOI: 10.1530/rep.0.1220677. View

11.

Jewgenow K, Neubauer K, Blottner S, Schon J, Wildt D, Pukazhenthi B . Reduced germ cell apoptosis during spermatogenesis in the teratospermic domestic cat. J Androl. 2009; 30(4):460-8. DOI: 10.2164/jandrol.108.006726. View

12.

Blottner S, Jewgenow K . Moderate seasonality in testis function of domestic cat. Reprod Domest Anim. 2007; 42(5):536-40. DOI: 10.1111/j.1439-0531.2006.00817.x. View

13.

Prochowska S, Nizanski W, Ochota M, Partyka A . Characteristics of urethral and epididymal semen collected from domestic cats--A retrospective study of 214 cases. Theriogenology. 2015; 84(9):1565-71. DOI: 10.1016/j.theriogenology.2015.08.005. View

14.

Tapanainen J, Tilly J, Vihko K, Hsueh A . Hormonal control of apoptotic cell death in the testis: gonadotropins and androgens as testicular cell survival factors. Mol Endocrinol. 1993; 7(5):643-50. DOI: 10.1210/mend.7.5.8316250. View

15.

Spindler R, Wildt D . Circannual variations in intraovarian oocyte but not epididymal sperm quality in the domestic Cat. Biol Reprod. 1999; 61(1):188-94. DOI: 10.1095/biolreprod61.1.188. View

16.

Print C, Loveland K . Germ cell suicide: new insights into apoptosis during spermatogenesis. Bioessays. 2000; 22(5):423-30. DOI: 10.1002/(SICI)1521-1878(200005)22:5<423::AID-BIES4>3.0.CO;2-0. View

17.

Blanco-Rodriguez J, Martinez-Garcia C . Apoptosis is physiologically restricted to a specialized cytoplasmic compartment in rat spermatids. Biol Reprod. 1999; 61(6):1541-7. DOI: 10.1095/biolreprod61.6.1541. View

18.

Siemieniuch M . Apoptotic changes in the epithelium germinativum of the cat (Felis catus s. domestica, L. 1758) at different ages and breeding seasons. Reprod Domest Anim. 2008; 43(4):473-6. DOI: 10.1111/j.1439-0531.2007.00937.x. View

19.

Cohen G . Caspases: the executioners of apoptosis. Biochem J. 1997; 326 ( Pt 1):1-16. PMC: 1218630. DOI: 10.1042/bj3260001. View

20.

Henriksen K, Kulmala J, Toppari J, Mehrotra K, Parvinen M . Stage-specific apoptosis in the rat seminiferous epithelium: quantification of irradiation effects. J Androl. 1996; 17(4):394-402. View