Identification of Selected Tuna Species in Commercial Products

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Mar 6

PMID 33672711

Citations 9

Authors

Eliska Servusova

Zora Piskata

Affiliations

Soon will be listed here.

Abstract

This study was conducted to develop systems for the identification of four tuna species (skipjack tuna , yellowfin tuna , bullet tuna sp. and Atlantic bonito sp). At first, raw samples of these species and a mix intended as internal control were prepared for the authentication of fish muscle tissue of the genus sp., sp. and sp. DNA from raw muscle tissue, the mix and samples was extracted with the DNeasy mericon Food Kit (Qiagen GmbH, Hilden, Germany). The concentration and purity of DNA in raw samples were evaluated using a spectrophotometer. Primers and probe sequences were specifically designed to identify the selected species. In addition, primers and a probe for the endogenous gene were designed to determine the presence of amplifiable fish (especially tuna) DNA in samples. Furthermore, the species specificity of the designed primers and probes was verified in DNA samples of various tuna and bonito species. Limit of detection for the selected species was calculated as well as the coefficient of determination and efficiency of real-time PCR testing was determined. To evaluate the developed real-time PCR methods, 70 commercial tuna products were analysed. The results show that mislabelling of fish products can still be encountered and, moreover, the presence of an additional species can be identified.

Citing Articles

Development of Single-Nucleotide Polymorphism (SNP)-Based Species-Specific Real-Time PCR Assays for Authenticating Five Highly Priced Tuna.

Qu M, Jiang Y, Guo Y, Zhu W, Li N, Zhao X Foods. 2024; 13(22).

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Molecular Rapid Test for Identification of Tuna Species.

Gkini I, Christopoulos P, Conides A, Kalogianni D, Christopoulos T Biosensors (Basel). 2024; 14(2).

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Development of a tetra-primer ARMS-PCR for identification of sika and red deer and their hybrids.

Ke-Xin Y, Xiang C, Qing-Qing H, Yi-An Y, Xiao-Ming W, Ai-Chun X Anal Sci. 2023; 39(12):1947-1956.

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Development and intralaboratory validation of three species using a multiplex polymerase chain reaction assay combined with capillary electrophoresis.

Lee G, Yang S, Kim H Food Sci Biotechnol. 2023; 32(10):1395-1404.

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A next-generation sequencing approach for the detection of mixed species in canned tuna.

Klapper R, Velasco A, Doring M, Schroder U, Sotelo C, Brinks E Food Chem X. 2023; 17:100560.

PMID: 36845509 PMC: 9943852. DOI: 10.1016/j.fochx.2023.100560.

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