Impact of In Vitro Gastrointestinal Digestion on the Bioaccessibility of Phytochemical Compounds from Eight Fruit Juices

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Mar 6

PMID 33672156

Citations 9

Authors

Dasha Mihaylova

Ivelina Desseva

Magdalena Stoyanova

Nadezhda Petkova

Margarita Terzyiska

Anna Lante

Affiliations

Soon will be listed here.

Abstract

Fruits contain a number of useful substances including antioxidants. Their bio-accessibility after passing through the digestive tract is of primary importance when considering their benefits. In this respect, we investigated the effect of in vitro digestion on the phytochemicals of eight fruit juices. Freshly prepared juices from pomegranate, orange and grapefruit were used as well as commercially available juices from cherry, black grapes and aloe vera, blackberry and chokeberry, and two types of chokeberry and raspberries. Spectrophotometric and HPLC methods were used in order to analyse the sugar content, the total phenolic (TPC) and flavonoid contents (TFC), anthocyanins, phenolic acids and antioxidant activity. Principle component analysis was used to explain the differentiation among the types of fruit juice. Sugar recovery variation was between 4-41%. The bio-accessibility of TPC ranged from 13.52-26.49% and of flavonoids between 24.25-67.00%. The pomegranate juice and the juice of black grapes and aloe vera kept 58.12 and 50.36% of their initial anthocyanins content, while for the other samples less than 1.10% was established. As a result, a maximum of 30% remaining antioxidant activity was measured for some of the samples, but for most this was less than 10%. In conclusion, fruit juices are a rich source of biologically active substances, but a more detailed analysis of food transformation during digestion is needed.

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References

Kay C . Aspects of anthocyanin absorption, metabolism and pharmacokinetics in humans. Nutr Res Rev. 2008; 19(1):137-46. DOI: 10.1079/NRR2005116. View

Zhang Q, Cheng Z, Wang Y, Fu L . Dietary protein-phenolic interactions: characterization, biochemical-physiological consequences, and potential food applications. Crit Rev Food Sci Nutr. 2020; 61(21):3589-3615. DOI: 10.1080/10408398.2020.1803199. View

Manach C, Williamson G, Morand C, Scalbert A, Remesy C . Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr. 2005; 81(1 Suppl):230S-242S. DOI: 10.1093/ajcn/81.1.230S. View

Brglez Mojzer E, Knez Hrncic M, Skerget M, Knez Z, Bren U . Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules. 2016; 21(7). PMC: 6273793. DOI: 10.3390/molecules21070901. View

Krinsky N . Mechanism of action of biological antioxidants. Proc Soc Exp Biol Med. 1992; 200(2):248-54. DOI: 10.3181/00379727-200-43429. View

Bao T, Wang Y, Li Y, Gowd V, Niu X, Yang H . Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion. J Zhejiang Univ Sci B. 2016; 17(12):941-951. PMC: 5172599. DOI: 10.1631/jzus.B1600243. View

Alminger M, Aura A, Bohn T, Dufour C, El S, Gomes A . In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility. Compr Rev Food Sci Food Saf. 2021; 13(4):413-436. DOI: 10.1111/1541-4337.12081. View

Seraglio S, Valese A, Daguer H, Bergamo G, Azevedo M, Nehring P . Effect of in vitro gastrointestinal digestion on the bioaccessibility of phenolic compounds, minerals, and antioxidant capacity of Mimosa scabrella Bentham honeydew honeys. Food Res Int. 2017; 99(Pt 1):670-678. DOI: 10.1016/j.foodres.2017.06.024. View

Grgic J, Selo G, Planinic M, Tisma M, Bucic-Kojic A . Role of the Encapsulation in Bioavailability of Phenolic Compounds. Antioxidants (Basel). 2020; 9(10). PMC: 7601682. DOI: 10.3390/antiox9100923. View

10.

Rodriguez-Roque M, Rojas-Grau M, Elez-Martinez P, Martin-Belloso O . Changes in vitamin C, phenolic, and carotenoid profiles throughout in vitro gastrointestinal digestion of a blended fruit juice. J Agric Food Chem. 2013; 61(8):1859-67. DOI: 10.1021/jf3044204. View

11.

Frank T, Stintzing F, Carle R, Bitsch I, Quaas D, Strass G . Urinary pharmacokinetics of betalains following consumption of red beet juice in healthy humans. Pharmacol Res. 2005; 52(4):290-7. DOI: 10.1016/j.phrs.2005.04.005. View

12.

Tenore G, Campiglia P, Ritieni A, Novellino E . In vitro bioaccessibility, bioavailability and plasma protein interaction of polyphenols from Annurca apple (M. pumila Miller cv Annurca). Food Chem. 2013; 141(4):3519-24. DOI: 10.1016/j.foodchem.2013.06.051. View

13.

Lachowicz S, Oszmianski J . The influence of addition of cranberrybush juice to pear juice on chemical composition and antioxidant properties. J Food Sci Technol. 2018; 55(9):3399-3407. PMC: 6098759. DOI: 10.1007/s13197-018-3233-8. View

14.

Mihaylova D, Vrancheva R, Desseva I, Ivanov I, Dincheva I, Popova M . Analysis of the GC-MS of volatile compounds and the phytochemical profile and antioxidant activities of some Bulgarian medicinal plants. Z Naturforsch C J Biosci. 2018; 74(1-2):45-54. DOI: 10.1515/znc-2018-0122. View

15.

Haida Z, Hakiman M . A comprehensive review on the determination of enzymatic assay and nonenzymatic antioxidant activities. Food Sci Nutr. 2019; 7(5):1555-1563. PMC: 6526636. DOI: 10.1002/fsn3.1012. View

16.

McDougall G, Dobson P, Smith P, Blake A, Stewart D . Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. J Agric Food Chem. 2005; 53(15):5896-904. DOI: 10.1021/jf050131p. View

17.

Brodkorb A, Egger L, Alminger M, Alvito P, Assuncao R, Ballance S . INFOGEST static in vitro simulation of gastrointestinal food digestion. Nat Protoc. 2019; 14(4):991-1014. DOI: 10.1038/s41596-018-0119-1. View

18.

Coe S, Fraser A, Ryan L . Polyphenol Bioaccessibility and Sugar Reducing Capacity of Black, Green, and White Teas. Int J Food Sci. 2016; 2013:238216. PMC: 4745553. DOI: 10.1155/2013/238216. View

19.

Mosele J, Macia A, Romero M, Motilva M . Stability and metabolism of Arbutus unedo bioactive compounds (phenolics and antioxidants) under in vitro digestion and colonic fermentation. Food Chem. 2016; 201:120-30. DOI: 10.1016/j.foodchem.2016.01.076. View

20.

Bohn T, Carriere F, Day L, Deglaire A, Egger L, Freitas D . Correlation between in vitro and in vivo data on food digestion. What can we predict with static in vitro digestion models?. Crit Rev Food Sci Nutr. 2017; 58(13):2239-2261. DOI: 10.1080/10408398.2017.1315362. View