Spread of Linezolid-Resistant Spp. in Human Clinical Isolates in the Czech Republic

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2021 Mar 6

PMID 33671753

Citations 10

Authors

Lucia Malisova

Vladislav Jakubu

Katarina Pomorska

Martin Musilek

Helena Zemlickova

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of and were examined in the National Reference Laboratory for Antibiotics. Among them, 8% of isolates ( = 115) were resistant to linezolid (/ = 106, / = 9). Only three strains of were resistant to tigecycline, 72.6% of isolates were resistant to vancomycin. One isolate of harbored the gene. The majority (87%, = 11) of strains were resistant to linezolid because of the mutation G2576T in the domain V of the 23S rRNA. This mutation was detected also in two strains of . The presence of the gene was the dominant mechanism of linezolid resistance in isolates. None of enterococci contained , genes, or any amino acid mutation in genes encoding ribosomal proteins. No mechanism of resistance was identified in 4 out of 106 linezolid resistant isolates in this study. Seventeen sequence types (STs) including four novel STs were identified in this work. Clonal complex CC17 was found in all isolates.

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