Versatile CRISPR/Cas9 Systems for Genome Editing in

Overview

Journal J Fungi (Basel)

Date 2021 Mar 6

PMID 33670568

Citations 6

Authors

Sarah-Maria Wege

Katharina Gejer

Fabienne Becker

Michael Bolker

Johannes Freitag

Bjorn Sandrock

Affiliations

Soon will be listed here.

Abstract

The phytopathogenic smut fungus is a versatile model organism to study plant pathology, fungal genetics, and molecular cell biology. Here, we report several strategies to manipulate the genome of by the CRISPR/Cas9 technology. These include targeted gene deletion via homologous recombination of short double-stranded oligonucleotides, introduction of point mutations, heterologous complementation at the genomic locus, and endogenous N-terminal tagging with the fluorescent protein mCherry. All applications are independent of a permanent selectable marker and only require transient expression of the endonuclease Cas9hf and sgRNA. The techniques presented here are likely to accelerate research in the community but can also act as a template for genome editing in other important fungi.

Citing Articles

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