Synthesis of Fluorescent Carbon Dots and Their Application in Ascorbic Acid Detection

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Mar 6

PMID 33669142

Citations 6

Authors

Tengfei Wang

Hui Luo

Xu Jing

Jiali Yang

Meijun Huo

Yu Wang

Affiliations

Soon will be listed here.

Abstract

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe composite was prepared by the interaction of the CDs solution and Fe solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0-350.0 μmol·L. Moreover, the detection limit was 3.11 μmol·L. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.

Citing Articles

-Derived Near-Infrared Carbon Dots for Bioimaging and Antimicrobial Applications.

Shi W, Li J, Pu J, Cheng G, Liu Y, Xiao S Molecules. 2025; 30(2).

PMID: 39860290 PMC: 11767839. DOI: 10.3390/molecules30020422.

Green synthetic natural carbon dots derived from with inhibitory effect against alcoholic gastric ulcer.

Zhao Y, Cheng G, Gao Y, Cui L, Zhao Y, Zhang Y Front Mol Biosci. 2023; 10:1223621.

PMID: 37484528 PMC: 10360179. DOI: 10.3389/fmolb.2023.1223621.

Progress in Fluorescence Biosensing and Food Safety towards Point-of-Detection (PoD) System.

Kakkar S, Gupta P, Kumar N, Kant K Biosensors (Basel). 2023; 13(2).

PMID: 36832016 PMC: 9953818. DOI: 10.3390/bios13020249.

One-Step Synthesis of Nitrogen/Fluorine Co-Doped Carbon Dots for Use in Ferric Ions and Ascorbic Acid Detection.

Zhao Y, Zhu X, Liu L, Duan Z, Liu Y, Zhang W Nanomaterials (Basel). 2022; 12(14).

PMID: 35889602 PMC: 9323265. DOI: 10.3390/nano12142377.

A facile imine-linked covalent organic framework doped with a carbon dot composite for the detection and removal of Hg in surface water.

Shili Q, Xudong H, Fenglong J, Ying W, Hongtao C, Shuang H RSC Adv. 2022; 12(29):18784-18793.

PMID: 35873319 PMC: 9237831. DOI: 10.1039/d2ra01236g.

References

Jana J, Lee H, Chung J, Kim M, Hur S . Blue emitting nitrogen-doped carbon dots as a fluorescent probe for nitrite ion sensing and cell-imaging. Anal Chim Acta. 2019; 1079:212-219. DOI: 10.1016/j.aca.2019.06.064. View

Zhu S, Meng Q, Wang L, Zhang J, Song Y, Jin H . Highly photoluminescent carbon dots for multicolor patterning, sensors, and bioimaging. Angew Chem Int Ed Engl. 2013; 52(14):3953-7. DOI: 10.1002/anie.201300519. View

Donate-Buendia C, Torres-Mendieta R, Pyatenko A, Falomir E, Fernandez-Alonso M, Minguez-Vega G . Fabrication by Laser Irradiation in a Continuous Flow Jet of Carbon Quantum Dots for Fluorescence Imaging. ACS Omega. 2018; 3(3):2735-2742. PMC: 6044845. DOI: 10.1021/acsomega.7b02082. View

Ugur H, Catak J, Mizrak O, Cebi N, Yaman M . Determination and evaluation of in vitro bioaccessibility of added vitamin C in commercially available fruit-, vegetable-, and cereal-based baby foods. Food Chem. 2020; 330:127166. DOI: 10.1016/j.foodchem.2020.127166. View

Shao B, Liu Z, Zeng G, Liu Y, Yang X, Zhou C . Immobilization of laccase on hollow mesoporous carbon nanospheres: Noteworthy immobilization, excellent stability and efficacious for antibiotic contaminants removal. J Hazard Mater. 2018; 362:318-326. DOI: 10.1016/j.jhazmat.2018.08.069. View

Zhu Y, He Y, Su T, Li C, Cai S, Wu Z . Exogenous vitamin C triggered structural changes of redox-activated dual core-crosslinked biodegradable nanogels for boosting the antitumor efficiency. J Mater Chem B. 2020; 8(23):5109-5116. DOI: 10.1039/d0tb00356e. View

Miyazawa T, Matsumoto A, Miyahara Y . Determination of cellular vitamin C dynamics by HPLC-DAD. Analyst. 2019; 144(11):3483-3487. DOI: 10.1039/c8an02240b. View

Zeng L, Li X, Fan S, Li J, Mu J, Qin M . The bioelectrochemical synthesis of high-quality carbon dots with strengthened electricity output and excellent catalytic performance. Nanoscale. 2019; 11(10):4428-4437. DOI: 10.1039/c8nr10510c. View

Pan D, Zhang J, Li Z, Wu M . Hydrothermal route for cutting graphene sheets into blue-luminescent graphene quantum dots. Adv Mater. 2010; 22(6):734-8. DOI: 10.1002/adma.200902825. View

10.

Ding Y, Gong X, Liu Y, Lu W, Gao Y, Xian M . Facile preparation of bright orange fluorescent carbon dots and the constructed biosensing platform for the detection of pH in living cells. Talanta. 2018; 189:8-15. DOI: 10.1016/j.talanta.2018.06.060. View

11.

Yi Z, Li X, Zhang H, Ji X, Sun W, Yu Y . High quantum yield photoluminescent N-doped carbon dots for switch sensing and imaging. Talanta. 2020; 222:121663. DOI: 10.1016/j.talanta.2020.121663. View

12.

Drehmer E, Navarro-Moreno M, Carrera S, Villar V, Moreno M . Oxygenic metabolism in nutritional obesity induced by olive oil. The influence of vitamin C. Food Funct. 2019; 10(6):3567-3580. DOI: 10.1039/c8fo02550a. View

13.

Ma X, Lin S, Dang Y, Dai Y, Zhang X, Xia F . Carbon dots as an "on-off-on" fluorescent probe for detection of Cu(II) ion, ascorbic acid, and acid phosphatase. Anal Bioanal Chem. 2019; 411(25):6645-6653. DOI: 10.1007/s00216-019-02038-z. View

14.

Kong L, Hu W, Lu C, Cheng K, Tang M . Mechanisms underlying nickel nanoparticle induced reproductive toxicity and chemo-protective effects of vitamin C in male rats. Chemosphere. 2018; 218:259-265. DOI: 10.1016/j.chemosphere.2018.11.128. View

15.

Luo X, Zhang W, Han Y, Chen X, Zhu L, Tang W . N,S co-doped carbon dots based fluorescent "on-off-on" sensor for determination of ascorbic acid in common fruits. Food Chem. 2018; 258:214-221. DOI: 10.1016/j.foodchem.2018.03.032. View

16.

Barati A, Shamsipur M, Abdollahi H . Hemoglobin detection using carbon dots as a fluorescence probe. Biosens Bioelectron. 2015; 71:470-475. DOI: 10.1016/j.bios.2015.04.073. View

17.

Kavitha T, Kumar S . Turning date palm fronds into biocompatible mesoporous fluorescent carbon dots. Sci Rep. 2018; 8(1):16269. PMC: 6214973. DOI: 10.1038/s41598-018-34349-z. View

18.

Yang W, Ni J, Luo F, Weng W, Wei Q, Lin Z . Cationic Carbon Dots for Modification-Free Detection of Hyaluronidase via an Electrostatic-Controlled Ratiometric Fluorescence Assay. Anal Chem. 2017; 89(16):8384-8390. DOI: 10.1021/acs.analchem.7b01705. View

19.

Yu Y, Li C, Chen C, Huang H, Liang C, Lou Y . Saccharomyces-derived carbon dots for biosensing pH and vitamin B 12. Talanta. 2019; 195:117-126. DOI: 10.1016/j.talanta.2018.11.010. View

20.

Gong X, Liu Y, Yang Z, Shuang S, Zhang Z, Dong C . An "on-off-on" fluorescent nanoprobe for recognition of chromium(VI) and ascorbic acid based on phosphorus/nitrogen dual-doped carbon quantum dot. Anal Chim Acta. 2017; 968:85-96. DOI: 10.1016/j.aca.2017.02.038. View