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An Improved Procedure for Background Correction in Autoradiography

Overview
Journal Histochemistry
Specialty Biochemistry
Date 1988 Jan 1
PMID 3366645
Citations 2
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Abstract

In the event of weak autoradiographic labelling, the proportion of truly labelled cells or structures can be calculated from the frequency distributions of grains per area or cell structure for i = 0, 1,..., n grains using the results obtained for an experimental group after the application of a radioactively labelled substance and those obtained for a control group without radioactivity. The principle of this computer-aided method is also applicable when the grain counts are related to varying areas in histological sections.

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