Distribution of Human Papillomavirus Genotypes in Women with High-grade Cervical Intraepithelial Lesions and Cervical Carcinoma and Analysis of Human Papillomavirus-16 Genomic Variants

Overview

Journal Croat Med J

Specialty General Medicine

Date 2021 Mar 4

PMID 33660963

Citations 4

Authors

Magdalena Karadza

Snjezana Zidovec Lepej

Ana Planinic

Ivana Grgic

Ante Corusic

Pavao Planinic

Mario Coric

Lea Hosnjak

Kristina Fujs Komlos

Mario Poljak

Adriana Vince

Affiliations

Soon will be listed here.

Abstract

Aim: To analyze the distribution of high-risk human papillomavirus (HR-HPV) genotypes and the diversity of HPV-16 genomic variants in Croatian women with high-grade squamous intraepithelial lesions (HSIL) and cervical carcinoma.

Methods: Tissue biopsy specimens were obtained from 324 women with histopathologically confirmed HSIL or cervical carcinoma, 5 women with low-grade SIL, and 49 women with negative histopathology. HR-HPV DNA was detected with Ampliquality HPV-type nucleic-acid hybridization assay, which identifies 29 different HPV genotypes. HPV-16 genomic variants were analyzed by an in-house sequencing.

Results: The most common HPV type in women with HSIL was HPV-16, detected in 127/219 (57.9%) specimens. HPV-16 was also the dominant type in squamous cell cervical carcinoma (46/69 or 66.7%) and in adenocarcinoma (18/36 or 50.0%). Out of 378 patients, 360 had HR-HPV (282 single infections and 79 multiple infections), 3 (0.8%) patients had low-risk HPV, and 15 (4%) tested negative. HPV-16 variants were determined in 130 HPV-16 positive specimens, including 74 HSIL and 46 carcinoma specimens. In HSIL specimens, 41 distinct variants were found, 98.6% belonging to the European branch and 1.4% belonging to the African branch. In cervical carcinoma specimens, 95% isolates grouped in 41 variants belonging to the European branch, one isolate (2.5%) belonged to the North American, and one (2.5%) to the Asian-American branch.

Conclusion: HPV-16, mainly belonging to the European branch, was the most frequent HPV genotype in women from Croatia with histologically confirmed HSIL and cervical cancer.

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