Combined LC-MS/MS and 16S RDNA Analysis on Mice Under High Temperature and Humidity and Herb Yinchen Protection Mechanism

Overview

Journal Sci Rep

Specialty Science

Date 2021 Mar 4

PMID 33658635

Citations 1

Authors

Yao Wang

Jiayi Chen

Jianbang Tang

Jiedong Xiao

Yuhua Zheng

Liting Tang

Huanhuan Luo

Affiliations

Soon will be listed here.

Abstract

With increased global warming, the impact of high temperature and humidity (HTH) on human health is increasing. Traditional Chinese medicine describes the Herb Yinchen as a remedy for reducing heat and eliminating dampness. This study focused on the impact of HTH conditions on mice and the potential protective effect of Herb Yinchen. Five male Balb/c mouse groups included two normal control groups, two HTH-exposed groups, and one Yinchen-treated group. For either three or ten days, normal and HTH-exposed mice were housed under normal or HTH (33 ± 2 °C,85% relative humidity) conditions, respectively. Yinchen-treated mice, housed under HTH conditions, received the Herb Yinchen decoction for three days. Metabolite profiles of plasma and liver samples from each group were analyzed using LC-MS/MS. Fecal DNA was extracted for 16S rDNA analysis to evaluate the intestinal microbiome. Spearman correlation analysis was performed on metabolites, bacteria, and bile acids that differed between the groups. We found that HTH altered the host metabolite profiles and reduced microbial diversity, causing intestinal microbiome imbalance. Interestingly, Herb Yinchen treatment improved HTH-mediated changes of the metabolite profiles and the intestinal microbiome, restoring them to values observed in normal controls. In conclusion, our study reveals that HTH causes intestinal bacterial disturbances and metabolic disorders in normal mice, while Herb Yinchen could afford protection against such changes.

Citing Articles

High temperature and humidity in the environment disrupt bile acid metabolism, the gut microbiome, and GLP-1 secretion in mice.

Chen S, Hu Z, Tang J, Zhu H, Zheng Y, Xiao J Commun Biol. 2024; 7(1):465.

PMID: 38632312 PMC: 11024098. DOI: 10.1038/s42003-024-06158-w.

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