Global Profiling of Distinct Cysteine Redox Forms Reveals Wide-ranging Redox Regulation in C. Elegans

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Mar 4

PMID 33658510

Citations 37

Authors

Jin Meng

Ling Fu

Keke Liu

Caiping Tian

Ziyun Wu

Youngeun Jung

Renan B Ferreira

Kate S Carroll

T Keith Blackwell

Jing Yang

Affiliations

Soon will be listed here.

Abstract

Post-translational changes in the redox state of cysteine residues can rapidly and reversibly alter protein functions, thereby modulating biological processes. The nematode C. elegans is an ideal model organism for studying cysteine-mediated redox signaling at a network level. Here we present a comprehensive, quantitative, and site-specific profile of the intrinsic reactivity of the cysteinome in wild-type C. elegans. We also describe a global characterization of the C. elegans redoxome in which we measured changes in three major cysteine redox forms after HO treatment. Our data revealed redox-sensitive events in translation, growth signaling, and stress response pathways, and identified redox-regulated cysteines that are important for signaling through the p38 MAP kinase (MAPK) pathway. Our in-depth proteomic dataset provides a molecular basis for understanding redox signaling in vivo, and will serve as a valuable and rich resource for the field of redox biology.

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