Preserving the Female Genome in Trehalose Glass at Supra-Zero Temperatures: The Relationship Between Moisture Content and DNA Damage in Feline Germinal Vesicles

Overview

Journal Cell Mol Bioeng

Publisher Springer

Specialty Biomedical Engineering

Date 2021 Mar 1

PMID 33643469

Citations 4

Authors

Shangping Wang

Pei-Chih Lee

Amanda Elsayed

Fan Zhang

Yong Zhang

Pierre Comizzoli

Gloria D Elliott

Affiliations

Soon will be listed here.

Abstract

Introduction: Maintaining a stable dry state is critical for long-term preservation of live biomaterials at suprazero temperatures. The objective of the study was to characterize the effect of moisture content on DNA integrity within the germinal vesicle (GV) of feline oocytes following dehydration and storage at 22-24 °C.

Methods: Using microwave-assisted drying, conditions that led to a predictable and stable moisture content in trehalose solutions were determined. To explore moisture content stability during storage, trehalose samples were dried for 15 min and stored in glass vials at 11 or 43% RH for 8 weeks. To examine whether this condition allowed proper storage of GVs, permeabilized cat oocytes were incubated in trehalose for 10 min and dried for 15 or 30 min. Oocytes then were rehydrated to assess DNA integrity either directly after drying or after 8 weeks of storage in an 11% RH environment. Raman spectroscopy was used to identify the states of dried samples during storage.

Results: Moisture content was stable during the storage period. There was no significant difference in DNA integrity between fresh and dried samples without storage. After 8 weeks of storage, DNA integrity was maintained in GVs dried for 30 min. Samples dried for 15 min and stored were compromised, suggesting crystallization of the preservation matrix during storage. Biostabilization was optimal when samples were directly processed to moisture contents consistent with storage in the glassy state.

Conclusion: Microwave-assisted drying processing and storage conditions were optimized to ensure stable long-term storage of structural and functional properties of genetic resources.

Citing Articles

Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles†.

Lee P, Comizzoli P Biol Reprod. 2024; 111(2):312-321.

PMID: 38637297 PMC: 11327313. DOI: 10.1093/biolre/ioae060.

The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes.

Orozco Cabral J, Lee P, Wang S, Wang Y, Zhang Y, Comizzoli P Bioengineering (Basel). 2023; 10(9).

PMID: 37760102 PMC: 10525460. DOI: 10.3390/bioengineering10091000.

Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?.

Comizzoli P, Loi P, Patrizio P, Hubel A J Assist Reprod Genet. 2022; 39(2):321-325.

PMID: 34984597 PMC: 8956754. DOI: 10.1007/s10815-021-02392-x.

Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations.

Brogna R, Fan J, Sieme H, Wolkers W, Oldenhof H Sci Rep. 2021; 11(1):14076.

PMID: 34234244 PMC: 8263733. DOI: 10.1038/s41598-021-93569-y.

References

Dang-Nguyen T, Nguyen H, Nguyen M, Somfai T, Noguchi J, Kaneko H . Maturation ability after transfer of freeze-dried germinal vesicles from porcine oocytes. Anim Sci J. 2018; 89(9):1253-1260. DOI: 10.1111/asj.13067. View

Patrick J, Elliott G, Comizzoli P . Structural integrity and developmental potential of spermatozoa following microwave-assisted drying in the domestic cat model. Theriogenology. 2017; 103:36-43. DOI: 10.1016/j.theriogenology.2017.07.037. View

Kotula A, Snyder C, Migler K . Determining conformational order and crystallinity in polycaprolactone via Raman spectroscopy. Polymer (Guildf). 2017; 117:1-10. PMC: 5557303. DOI: 10.1016/j.polymer.2017.04.006. View

Comizzoli P, Pukazhenthi B, Wildt D . The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model. Hum Reprod. 2011; 26(8):2165-77. PMC: 3137393. DOI: 10.1093/humrep/der176. View

Deegan , Bakajin , DUPONT , HUBER , Nagel , Witten . Contact line deposits in an evaporating drop. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 2000; 62(1 Pt B):756-65. DOI: 10.1103/physreve.62.756. View

Elliott G, Wang S, Fuller B . Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology. 2017; 76:74-91. DOI: 10.1016/j.cryobiol.2017.04.004. View

Zhang J, Zografi G . The relationship between "BET" and "free volume"-derived parameters for water vapor absorption into amorphous solids. J Pharm Sci. 2000; 89(8):1063-72. DOI: 10.1002/1520-6017(200008)89:8<1063::aid-jps11>3.0.co;2-0. View

Chakravarty P, Bhardwaj S, King L, Suryanarayanan R . Monitoring phase transformations in intact tablets of trehalose by FT-Raman spectroscopy. AAPS PharmSciTech. 2009; 10(4):1420-6. PMC: 2799603. DOI: 10.1208/s12249-009-9337-8. View

Mazzobre M, Longinotti M, Corti H, Buera M . Effect of salts on the properties of aqueous sugar systems, in relation to biomaterial stabilization. 1. Water sorption behavior and ice crystallization/melting. Cryobiology. 2002; 43(3):199-210. DOI: 10.1006/cryo.2001.2345. View

10.

Chakraborty N, Menze M, Malsam J, Aksan A, Hand S, Toner M . Cryopreservation of spin-dried mammalian cells. PLoS One. 2011; 6(9):e24916. PMC: 3178566. DOI: 10.1371/journal.pone.0024916. View

11.

Sitaula R, Bhowmick S . Moisture sorption characteristics and thermophysical properties of trehalose-PBS mixtures. Cryobiology. 2006; 52(3):369-85. DOI: 10.1016/j.cryobiol.2006.01.006. View

12.

Elliott G, Lee P, Paramore E, Van Vorst M, Comizzoli P . Resilience of oocyte germinal vesicles to microwave-assisted drying in the domestic cat model. Biopreserv Biobank. 2015; 13(3):164-71. PMC: 4559202. DOI: 10.1089/bio.2014.0078. View

13.

Millqvist-Fureby A, Malmsten M, Bergenstahl B . Spray-drying of trypsin - surface characterisation and activity preservation. Int J Pharm. 1999; 188(2):243-53. DOI: 10.1016/s0378-5173(99)00226-4. View

14.

Crowe J, Carpenter J, Crowe L . The role of vitrification in anhydrobiosis. Annu Rev Physiol. 1998; 60:73-103. DOI: 10.1146/annurev.physiol.60.1.73. View

15.

Chen C . Pregnancy after human oocyte cryopreservation. Lancet. 1986; 1(8486):884-6. DOI: 10.1016/s0140-6736(86)90989-x. View

16.

Comizzoli P, Wildt D, Pukazhenthi B . Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. Mol Reprod Dev. 2007; 75(2):345-54. PMC: 2167628. DOI: 10.1002/mrd.20769. View

17.

Wood T, Wildt D . Effect of the quality of the cumulus-oocyte complex in the domestic cat on the ability of oocytes to mature, fertilize and develop into blastocysts in vitro. J Reprod Fertil. 1997; 110(2):355-60. DOI: 10.1530/jrf.0.1100355. View

18.

Chen T, Fowler A, Toner M . Literature review: supplemented phase diagram of the trehalose-water binary mixture. Cryobiology. 2000; 40(3):277-82. DOI: 10.1006/cryo.2000.2244. View

19.

Chakraborty N, Biswas D, Parker W, Moyer P, Elliott G . A role for microwave processing in the dry preservation of mammalian cells. Biotechnol Bioeng. 2008; 100(4):782-96. DOI: 10.1002/bit.21801. View

20.

Graves-Herring J, Wildt D, Comizzoli P . Retention of structure and function of the cat germinal vesicle after air-drying and storage at suprazero temperature. Biol Reprod. 2013; 88(6):139. PMC: 4070864. DOI: 10.1095/biolreprod.113.108472. View