Promises and Pitfalls of Parasite Patch-clamp

Overview

Journal Trends Parasitol

Specialty Parasitology

Date 2021 Feb 28

PMID 33640269

Citations 10

Authors

Jeanine Gezelle

Gagandeep Saggu

Sanjay A Desai

Affiliations

Soon will be listed here.

Abstract

Protozoan parasites acquire essential ions, nutrients, and other solutes from their insect and vertebrate hosts by transmembrane uptake. For intracellular stages, these solutes must cross additional membranous barriers. At each step, ion channels and transporters mediate not only this uptake but also the removal of waste products. These transport proteins are best isolated and studied with patch-clamp, but these methods remain accessible to only a few parasitologists due to specialized instrumentation and the required training in both theory and practice. Here, we provide an overview of patch-clamp, describing the advantages and limitations of the technology and highlighting issues that may lead to incorrect conclusions. We aim to help non-experts understand and critically assess patch-clamp data in basic research studies.

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