Comparative Genomics of MRSA Strains from Human and Canine Origins Reveals Similar Virulence Gene Repertoire

Overview

Journal Sci Rep

Specialty Science

Date 2021 Feb 26

PMID 33633263

Citations 12

Authors

Bruno Penna

Marcella B Silva

Andre E R Soares

Ana T R Vasconcelos

Mariana S Ramundo

Fabienne A Ferreira

Maria C Silva-Carvalho

Viviane S de Sousa

Renata F Rabello

Paula T Bandeira

Viviane S de Souza

Paul J Planet

Olney Vieira-da-Motta

Ana M N Botelho

Agnes M S Figueiredo

Affiliations

Soon will be listed here.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with a wide variety of infections in humans. The ability of MRSA to infect companion animals has gained increasing attention in the scientific literature. In this study, 334 dogs were screened for MRSA in two cities located in Rio de Janeiro State. The prevalence of MRSA in dogs was 2.7%. Genotyping revealed isolates from sequence types (ST) 1, 5, 30, and 239 either colonizing or infecting dogs. The genome of the canine ST5 MRSA (strain SA112) was compared with ST5 MRSA from humans-the main lineage found in Rio de Janeiro hospitals-to gain insights in the origin of this dog isolate. Phylogenetic analysis situated the canine genome and human strain CR14-035 in the same clade. Comparative genomics revealed similar virulence profiles for SA112 and CR14-035. Both genomes carry S. aureus genomic islands νSAα, νSAβ, and νSAγ. The virulence potential of the canine and human strains was similar in a Caenorhabditis elegans model. Together, these results suggest a potential of canine MRSA to infect humans and vice versa. The circulation in community settings of a MRSA lineage commonly found in hospitals is an additional challenge for public health surveillance authorities.

Citing Articles

Comparative genome analyses of Staphylococcus aureus from platelet concentrates reveal rearrangements involving loss of type VII secretion genes.

Ighem Chi S, Flint A, Weedmark K, Pagotto F, Ramirez-Arcos S Access Microbiol. 2024; 6(9).

PMID: 39697362 PMC: 11652724. DOI: 10.1099/acmi.0.000820.v4.

Methicillin-Resistant Carrying the PVL and Toxic Shock Syndrome Toxin in Healthy Dogs in Algeria.

Khermouche F, Heleili N, Merradi M, Hachemi A, Drapeau A, Murri S Antibiotics (Basel). 2024; 13(11).

PMID: 39596783 PMC: 11590900. DOI: 10.3390/antibiotics13111090.

Methicillin-resistant prevalence in food-producing animals and food products in Saudi Arabia: A review.

Alkuraythi D, Alkhulaifi M Vet World. 2024; 17(8):1753-1764.

PMID: 39328450 PMC: 11422649. DOI: 10.14202/vetworld.2024.1753-1764.

The Epidemiology of Animal-Associated Methicillin-Resistant .

Kasela M, Ossowski M, Dzikon E, Ignatiuk K, Wlazlo L, Malm A Antibiotics (Basel). 2023; 12(6).

PMID: 37370398 PMC: 10295410. DOI: 10.3390/antibiotics12061079.

Pet animals as reservoirs for spreading methicillin-resistant to human health.

Khairullah A, Sudjarwo S, Effendi M, Ramandinianto S, Gelolodo M, Widodo A J Adv Vet Anim Res. 2023; 10(1):1-13.

PMID: 37155545 PMC: 10122942. DOI: 10.5455/javar.2023.j641.

References

David M, Daum R . Community-associated methicillin-resistant Staphylococcus aureus: epidemiology and clinical consequences of an emerging epidemic. Clin Microbiol Rev. 2010; 23(3):616-87. PMC: 2901661. DOI: 10.1128/CMR.00081-09. View

Pardos de la Gandara M, Curry M, Berger J, Burstein D, Della-Latta P, Kopetz V . MRSA Causing Infections in Hospitals in Greater Metropolitan New York: Major Shift in the Dominant Clonal Type between 1996 and 2014. PLoS One. 2016; 11(6):e0156924. PMC: 4896443. DOI: 10.1371/journal.pone.0156924. View

Penna B, Varges R, Medeiros L, Martins G, Martins R, Lilenbaum W . Species distribution and antimicrobial susceptibility of staphylococci isolated from canine otitis externa. Vet Dermatol. 2010; 21(3):292-6. DOI: 10.1111/j.1365-3164.2009.00842.x. View

Lin Y, Barker E, Kislow J, Kaldhone P, Stemper M, Pantrangi M . Evidence of multiple virulence subtypes in nosocomial and community-associated MRSA genotypes in companion animals from the upper midwestern and northeastern United States. Clin Med Res. 2010; 9(1):7-16. PMC: 3064756. DOI: 10.3121/cmr.2010.944. View

Quitoco I, Ramundo M, Silva-Carvalho M, Souza R, Beltrame C, Oliveira T . First report in South America of companion animal colonization by the USA1100 clone of community-acquired meticillin-resistant Staphylococcus aureus (ST30) and by the European clone of methicillin-resistant Staphylococcus pseudintermedius (ST71). BMC Res Notes. 2013; 6:336. PMC: 3765899. DOI: 10.1186/1756-0500-6-336. View

Davis J, Jackson C, Fedorka-Cray P, Barrett J, Brousse J, Gustafson J . Carriage of methicillin-resistant staphylococci by healthy companion animals in the US. Lett Appl Microbiol. 2014; 59(1):1-8. DOI: 10.1111/lam.12254. View

van Duijkeren E, Moleman M, Sloet van Oldruitenborgh-Oosterbaan M, Multem J, Troelstra A, Fluit A . Methicillin-resistant Staphylococcus aureus in horses and horse personnel: an investigation of several outbreaks. Vet Microbiol. 2009; 141(1-2):96-102. DOI: 10.1016/j.vetmic.2009.08.009. View

Feingold B, Silbergeld E, Curriero F, van Cleef B, Heck M, Kluytmans J . Livestock density as risk factor for livestock-associated methicillin-resistant Staphylococcus aureus, the Netherlands. Emerg Infect Dis. 2012; 18(11):1841-9. PMC: 3559158. DOI: 10.3201/eid1811.111850. View

Manian F . Asymptomatic nasal carriage of mupirocin-resistant, methicillin-resistant Staphylococcus aureus (MRSA) in a pet dog associated with MRSA infection in household contacts. Clin Infect Dis. 2003; 36(2):e26-8. DOI: 10.1086/344772. View

10.

Nienhoff U, Kadlec K, Chaberny I, Verspohl J, Gerlach G, Schwarz S . Transmission of methicillin-resistant Staphylococcus aureus strains between humans and dogs: two case reports. J Antimicrob Chemother. 2009; 64(3):660-2. DOI: 10.1093/jac/dkp243. View

11.

Chamon R, Ribeiro S, da Costa T, Nouer S, Dos Santos K . Complete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil. Braz J Infect Dis. 2016; 21(2):185-189. PMC: 9427584. DOI: 10.1016/j.bjid.2016.09.015. View

12.

Kuroda M, Ohta T, Uchiyama I, Baba T, Yuzawa H, Kobayashi I . Whole genome sequencing of meticillin-resistant Staphylococcus aureus. Lancet. 2001; 357(9264):1225-40. DOI: 10.1016/s0140-6736(00)04403-2. View

13.

Hiramatsu K, Aritaka N, Hanaki H, Kawasaki S, Hosoda Y, Hori S . Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet. 1997; 350(9092):1670-3. DOI: 10.1016/S0140-6736(97)07324-8. View

14.

Coelho L, Souza R, Ferreira F, Guimaraes M, Ferreira-Carvalho B, Figueiredo A . agr RNAIII divergently regulates glucose-induced biofilm formation in clinical isolates of Staphylococcus aureus. Microbiology (Reading). 2008; 154(Pt 11):3480-3490. DOI: 10.1099/mic.0.2007/016014-0. View

15.

Gong J, Li D, Yan J, Liu Y, Li D, Dong J . The accessory gene regulator (agr) controls Staphylococcus aureus virulence in a murine intracranial abscesses model. Braz J Infect Dis. 2014; 18(5):501-6. PMC: 9428208. DOI: 10.1016/j.bjid.2014.03.005. View

16.

Pollitt E, West S, Crusz S, Burton-Chellew M, Diggle S . Cooperation, quorum sensing, and evolution of virulence in Staphylococcus aureus. Infect Immun. 2013; 82(3):1045-51. PMC: 3957977. DOI: 10.1128/IAI.01216-13. View

17.

Ishihara K, Saito M, Shimokubo N, Muramatsu Y, Maetani S, Tamura Y . Methicillin-resistant Staphylococcus aureus carriage among veterinary staff and dogs in private veterinary clinics in Hokkaido, Japan. Microbiol Immunol. 2014; 58(3):149-54. DOI: 10.1111/1348-0421.12128. View

18.

Jang Y, Bae D, Cho J, Bahk G, Lim S, Lee Y . Characterization of methicillin-resistant Staphylococcus spp. isolated from dogs in Korea. Jpn J Vet Res. 2015; 62(4):163-70. View

19.

Vincze S, Stamm I, Kopp P, Hermes J, Adlhoch C, Semmler T . Alarming proportions of methicillin-resistant Staphylococcus aureus (MRSA) in wound samples from companion animals, Germany 2010-2012. PLoS One. 2014; 9(1):e85656. PMC: 3896405. DOI: 10.1371/journal.pone.0085656. View

20.

Iverson S, Brazil A, Ferguson J, Nelson K, Lautenbach E, Rankin S . Anatomical patterns of colonization of pets with staphylococcal species in homes of people with methicillin-resistant Staphylococcus aureus (MRSA) skin or soft tissue infection (SSTI). Vet Microbiol. 2015; 176(1-2):202-8. DOI: 10.1016/j.vetmic.2015.01.003. View