Identification of Pathogenic Genes in Campylobacter Jejuni Isolated from Broiler Carcasses and Broiler Slaughterhouses

Overview

Journal Sci Rep

Specialty Science

Date 2021 Feb 26

PMID 33633256

Citations 14

Authors

Yuli Melisa Sierra-Arguello

Gustavo Perdoncini

Laura Beatriz Rodrigues

Luciana Ruschel Dos Santos

Karen Apellanis Borges

Thales Quedi Furian

Carlos Tadeu Pippi Salle

Hamilton Luiz de Souza Moraes

Marcos Jose Pereira Gomes

Vladimir Pinheiro do Nascimento

Affiliations

Soon will be listed here.

Abstract

Campylobacter jejuni is one of the most common causes of foodborne diseases worldwide. There are few reports on Campylobacter strains isolated from Latin-American countries. Here, 140 C. jejuni strains isolated from cloacal and transport boxes swabs, water from chiller tanks, and broiler carcasses of five poultry companies in Southern Brazil were identified using phenotypic and genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight C. jejuni virulence markers: flaA, cadF, and invasion-associated (iam) genes, cdtABC operon (associated with the cytolethal distending toxin), and plasmidial virB11 and wlaN genes were present in 78.5%, 77.8%, 0%, 74.2%, 22.1%, and 10.7% of samples, respectively. There were 25 different virulence profiles: 1 (cdtA, cdtB, cdtC, flaA, and cadF), 2 (cdtA, cdtB, cdtC, flaA, cadF, and virB11), and 3 (cdtA, cdtB, cdtC, flaA, cadF, and wlaN) were the most common (> 60% of strains). We provide insight into factors related to the occurrence of this pathogen and their epidemiology.

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