Expression Analysis of Tissue Factor Pathway Inhibitors TFPI-1 and TFPI-2 in Paralichthys Olivaceus and Antibacterial and Anticancer Activity of Derived Peptides

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2021 Feb 26

PMID 33632337

Citations 7

Authors

Guanghua Wang

Bing Xie

Yanli Su

Qinqin Gu

Dongfang Hao

Hongmei Liu

Changbiao Wang

Yonghua Hu

Min Zhang

Affiliations

Soon will be listed here.

Abstract

Tissue factor pathway inhibitors (TFPI), including TFPI-1 and TFPI-2, are Kunitz-type serine protease inhibitors that mainly inhibit the blood coagulation induced by tissue factors. Previous reports on teleost proved TFPI play important roles in innate immunity. In this study, two TFPI (PoTFPI-1 and PoTFPI-2) molecules from Japanese flounder (Paralichthys olivaceus) were analyzed and characterized for their expression patterns, antibacterial and anticancer activities of the C-terminal derived peptides. Quantitative real time RT-PCR analysis shows that constitutive PoTFPI-1 expression occurred, in increasing order, in the brain, muscle, spleen, gills, head kidney, blood, intestine, heart, and liver; PoTFPI-2 was expressed, in increasing order, in the brain, gills, head kidney, muscle, intestine, spleen, liver, heart, and blood. Under the stimulation of fish pathogens, both PoTFPI-1 and PoTFPI-2 expressions increased significantly in a manner that depended on the pathogens, tissue type, and infection stage. Furthermore, C-terminal peptides TP25 and TP26, derived from PoTFPI-1 and PoTFPI-2, respectively, were synthesized and proved to be active against Micrococcus luteus (for TP25 and TP26) and Staphylococcus aureus (for TP25) via retardation effects on bacterial nucleic acids. In addition, TP25 and TP26 also displayed significant inhibitory effects on human colon cancer cell line HT-29. These results reveal that both PoTFPI-1 and PoTFPI-2 play important roles in host innate immunity. The antibacterial activity and anticancer cells function of TP25 and TP26 will add new insights into the roles of teleost TFPI.

Citing Articles

A novel family of defensin-like peptides from Hermetia illucens with antibacterial properties.

Fahmy L, Generalovic T, Ali Y, Seilly D, Sivanesan K, Kalmar L BMC Microbiol. 2024; 24(1):167.

PMID: 38755524 PMC: 11097590. DOI: 10.1186/s12866-024-03325-1.

Long Non-Coding RNAs: Bridging Cancer-Associated Thrombosis and Clinical Outcome of Ovarian Cancer Patients.

Marques I, Tavares V, Savva-Bordalo J, Rei M, Liz-Pimenta J, de Melo I Int J Mol Sci. 2024; 25(1).

PMID: 38203310 PMC: 10778953. DOI: 10.3390/ijms25010140.

Tuna trimmings hydrolysate alleviates immune stress and intestinal mucosal injury during chemotherapy on mice and identification of potentially active peptides.

Zhao X, Cai B, Chen H, Wan P, Chen D, Ye Z Curr Res Food Sci. 2023; 7:100547.

PMID: 37522134 PMC: 10371818. DOI: 10.1016/j.crfs.2023.100547.

Tissue Factor Pathway Inhibitors as Potential Targets for Understanding the Pathophysiology of Preeclampsia.

Kobayashi H, Matsubara S, Yoshimoto C, Shigetomi H, Imanaka S Biomedicines. 2023; 11(5).

PMID: 37238908 PMC: 10215522. DOI: 10.3390/biomedicines11051237.

Integrative analyses of RNA-seq and ChIP-seq Reveal MITF as a Target Gene of TFPI-2 in MDA231 Cells.

Wang G, Zhang G, Zhu N, Zhu C, Kang M, Zuo G Biochem Genet. 2023; 61(5):1745-1757.

PMID: 36787085 DOI: 10.1007/s10528-023-10340-w.

References

Zhang M, Li M, Sun L . NKLP27: a teleost NK-lysin peptide that modulates immune response, induces degradation of bacterial DNA, and inhibits bacterial and viral infection. PLoS One. 2014; 9(9):e106543. PMC: 4152322. DOI: 10.1371/journal.pone.0106543. View

Zhang Y, Wang L, Zhou W, Wang H, Zhang J, Deng S . Tissue factor pathway inhibitor-2: a novel gene involved in zebrafish central nervous system development. Dev Biol. 2013; 381(1):38-49. DOI: 10.1016/j.ydbio.2013.06.018. View

Sierko E, Wojtukiewicz M, Kisiel W . The role of tissue factor pathway inhibitor-2 in cancer biology. Semin Thromb Hemost. 2007; 33(7):653-9. DOI: 10.1055/s-2007-991532. View

Chand H, Foster D, Kisiel W . Structure, function and biology of tissue factor pathway inhibitor-2. Thromb Haemost. 2006; 94(6):1122-30. DOI: 10.1160/TH05-07-0509. View

Yang D, Zou R, Zhu Y, Liu B, Yao D, Jiang J . Magainin II modified polydiacetylene micelles for cancer therapy. Nanoscale. 2014; 6(24):14772-83. DOI: 10.1039/c4nr04405c. View

Iino M, Foster D, Kisiel W . Quantification and characterization of human endothelial cell-derived tissue factor pathway inhibitor-2. Arterioscler Thromb Vasc Biol. 1998; 18(1):40-6. DOI: 10.1161/01.atv.18.1.40. View

Miyagi Y, Koshikawa N, Yasumitsu H, Miyagi E, Hirahara F, Aoki I . cDNA cloning and mRNA expression of a serine proteinase inhibitor secreted by cancer cells: identification as placental protein 5 and tissue factor pathway inhibitor-2. J Biochem. 1994; 116(5):939-42. DOI: 10.1093/oxfordjournals.jbchem.a124648. View

Hisaka T, Lardeux B, Lamireau T, Wuestefeld T, Lalor P, Neaud V . Expression of tissue factor pathway inhibitor-2 in murine and human liver regulation during inflammation. Thromb Haemost. 2004; 91(3):569-75. DOI: 10.1160/TH03-06-0358. View

Aharon A, Brenner B, Katz T, Miyagi Y, Lanir N . Tissue factor and tissue factor pathway inhibitor levels in trophoblast cells: implications for placental hemostasis. Thromb Haemost. 2004; 92(4):776-86. DOI: 10.1160/TH04-01-0033. View

10.

Schirm S, Liu X, Jennings L, Jedrzejewski P, Dai Y, Hardy S . Fragmented tissue factor pathway inhibitor (TFPI) and TFPI C-terminal peptides eliminate serum-resistant Escherichia coli from blood cultures. J Infect Dis. 2009; 199(12):1807-15. DOI: 10.1086/599097. View

11.

Udagawa K, Miyagi Y, Hirahara F, Miyagi E, Nagashima Y, MINAGUCHI H . Specific expression of PP5/TFPI2 mRNA by syncytiotrophoblasts in human placenta as revealed by in situ hybridization. Placenta. 1998; 19(2-3):217-23. DOI: 10.1016/s0143-4004(98)90011-x. View

12.

Pawlinski R, Pedersen B, Schabbauer G, Tencati M, Holscher T, Boisvert W . Role of tissue factor and protease-activated receptors in a mouse model of endotoxemia. Blood. 2003; 103(4):1342-7. PMC: 2860856. DOI: 10.1182/blood-2003-09-3051. View

13.

Eliassen L, Berge G, Leknessund A, Wikman M, Lindin I, Lokke C . The antimicrobial peptide, lactoferricin B, is cytotoxic to neuroblastoma cells in vitro and inhibits xenograft growth in vivo. Int J Cancer. 2006; 119(3):493-500. DOI: 10.1002/ijc.21886. View

14.

Matyal R, Vin Y, Delude R, Lee C, Creasey A, Fink M . Extremely low doses of tissue factor pathway inhibitor decrease mortality in a rabbit model of septic shock. Intensive Care Med. 2001; 27(8):1274-80. DOI: 10.1007/s001340100992. View

15.

Zhao X, He S, Yue B, Wang G, Zhang M . Molecular characterization, expression analysis, and bactericidal activity of the derivative peptides of TFPI-1 and TFPI-2 in half-smooth tongue sole, Cynoglossus semilaevis. Fish Shellfish Immunol. 2016; 58:563-571. DOI: 10.1016/j.fsi.2016.10.003. View

16.

Lwaleed B, Bass P . Tissue factor pathway inhibitor: structure, biology and involvement in disease. J Pathol. 2005; 208(3):327-39. DOI: 10.1002/path.1871. View

17.

Sprecher C, Kisiel W, Mathewes S, Foster D . Molecular cloning, expression, and partial characterization of a second human tissue-factor-pathway inhibitor. Proc Natl Acad Sci U S A. 1994; 91(8):3353-7. PMC: 43575. DOI: 10.1073/pnas.91.8.3353. View

18.

Herman M, Sukhova G, Kisiel W, Foster D, Kehry M, Libby P . Tissue factor pathway inhibitor-2 is a novel inhibitor of matrix metalloproteinases with implications for atherosclerosis. J Clin Invest. 2001; 107(9):1117-26. PMC: 209273. DOI: 10.1172/JCI10403. View

19.

Fu X, Li N, Lai Y, Luo X, Wang Y, Shi C . A novel fish cell line derived from the brain of Chinese perch Siniperca chuatsi: development and characterization. J Fish Biol. 2014; 86(1):32-45. DOI: 10.1111/jfb.12540. View

20.

Crawley J, Lane D . The haemostatic role of tissue factor pathway inhibitor. Arterioscler Thromb Vasc Biol. 2007; 28(2):233-42. DOI: 10.1161/ATVBAHA.107.141606. View