Identification of Microalgae Cultured in Bold's Basal Medium from Freshwater Samples, from a High-rise City

Overview

Journal Sci Rep

Specialty Science

Date 2021 Feb 25

PMID 33627771

Citations 2

Authors

Charmaine Lloyd

Kai Heng Tan

Kar Leong Lim

Vimala Gana Valu

Sarah Mei Ying Fun

Teng Rong Chye

Hui Min Mak

Wei Xiong Sim

Sarah Liyana Musa

Joscelyn Jun Quan Ng

Nazurah Syazana Bte Nordin

Nurhazlyn Bte Md Aidzil

Zephyr Yu Wen Eng

Punithavathy Manickavasagam

Jen Yan New

Affiliations

Soon will be listed here.

Abstract

This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, E = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.

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