Anti-5'-Nucleotidases (5'-ND) and Acetylcholinesterase (AChE) Activities of Medicinal Plants to Combat Venom-Induced Toxicities

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2021 Feb 22

PMID 33614782

Citations 1

Authors

Nazia Aslam

Syeda Fatima

Sofia Khalid

Shahzad Hussain

Mughal Qayum

Khurram Afzal

Muhammad Hassham Hassan Bin Asad

Affiliations

Soon will be listed here.

Abstract

is one of the highly venomous snakes of Pakistan that is responsible for numerous cases of envenomation and deaths. In Pakistan, medicinal plants are commonly used traditionally for snakebite treatment because of their low cost and easy availability in comparison with antivenom. The current research is aimed at evaluating the inhibitory activity of Pakistani medicinal plants against acetylcholinesterase and 5'-nucleotidases present in venom. Acetylcholinesterase and 5'-nucleotidase enzymatic assays were performed at different venom concentrations to check the activity of these enzymes. Methanolic extracts from different parts of plants were used for in vitro determination of their inhibitory activity against 5'-nucleotidases in snake venom. Active methanolic extracts were subsequently fractioned using different solvents, and these fractions were also assessed for their anti-5'-nucleotidase activity. Results of this study exhibited that Willd. ex O. Berg, L., (L.) R. Br., (Wild.) R. Br., L., and L. were able to significantly ( > 0.5) neutralize the 5'-nucleotidase activity by 88%, 86%, 86%, 85%, 83.7%, and 83%, respectively, compared with a standard antidote (snake venom antiserum). Thus, this study indicates that these plants possess the potential to neutralize one of the toxic enzymatic components of venom and hence can help to augment the future efforts of developing alternative therapy for the management of snakebites.

Citing Articles

A Contemporary Exploration of Traditional Indian Snake Envenomation Therapies.

Deshpande A, Sastry K, Bhise S Trop Med Infect Dis. 2022; 7(6).

PMID: 35736986 PMC: 9227218. DOI: 10.3390/tropicalmed7060108.

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