Antibiofilm Activity of Small-Molecule ZY-214-4 Against

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Feb 22

PMID 33613488

Citations 5

Authors

Jingyi Yu

Lulin Rao

Lingling Zhan

Yan Zhou

Yinjuan Guo

Xiaocui Wu

Zengqiang Song

Fangyou Yu

Affiliations

Soon will be listed here.

Abstract

is the most important pathogenic bacteria in humans. As the resistance of to existing antibiotics is increasing, there is an urgent need for new anti-infective drugs. biofilms cause persistent infections and resist complete eradication with antibiotic therapy. The present study investigated the inhibitory effect of the novel small-molecule ZY-214-4 (C H BrNO) on biofilm formation. At a subinhibitory concentration (4 μg/ml), ZY-214-4 had no effect on the growth of strains and also showed no cytotoxicity in human normal bronchial epithelial cells (Bease-2B). The results of a semi-quantitative biofilm test showed that ZY-214-4 prevented biofilm formation, which was confirmed by scanning electron microscopy and confocal laser scanning microscopy. ZY-214-4 significantly suppressed the production of polysaccharide intercellular adhesion and prevented cell aggregation, and also inhibited the mRNA expression of and other biofilm-related genes (, , , , , α, and β) in clinical isolates. Thus, at a subinhibitory concentration, ZY-214-4 inhibits biofilm formation by preventing cell aggregation, highlighting its clinical potential for preventing or treating infections.

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