Molecular Characterization of Ascaridia Galli from Bangladesh and Development of a PCR Method for Distinguishing A. Galli from Heterakis Spp

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2021 Feb 22

PMID 33612660

Citations 1

Authors

Peru Gopal Biswas

Yuma Ohari

Uday Kumar Mohanta

Tadashi Itagaki

Affiliations

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Abstract

We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future.

Citing Articles

Ascaridia galli, a common nematode in semiscavenging indigenous chickens in Bangladesh: epidemiology, genetic diversity, pathobiology, ex vivo culture, and anthelmintic efficacy.

Ritu S, Labony S, Hossain M, Ali M, Mehedi Hasan M, Nadia N Poult Sci. 2024; 103(3):103405.

PMID: 38183880 PMC: 10809094. DOI: 10.1016/j.psj.2023.103405.

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