Mapping RRNA 2'-O-methylations and Identification of C/D SnoRNAs in Arabidopsis Thaliana Plants

Overview

Journal RNA Biol

Specialty Molecular Biology

Date 2021 Feb 18

PMID 33596769

Citations 20

Authors

J Azevedo-Favory

C Gaspin

L Ayadi

C Montacie

V Marchand

E Jobet

M Rompais

C Carapito

Y Motorin

J Saez-Vasquez

Affiliations

Soon will be listed here.

Abstract

In all eukaryotic cells, the most abundant modification of ribosomal RNA (rRNA) is methylation at the ribose moiety (2'--methylation). Ribose methylation at specific rRNA sites is guided by small nucleolar RNAs (snoRNAs) of C/D-box type (C/D snoRNA) and achieved by the methyltransferase Fibrillarin (FIB). Here we used the Illumina-based RiboMethSeq approach for mapping rRNA 2'--methylation sites in Col-0 (WT) plants. This analysis detected novel C/D snoRNA-guided rRNA 2'--methylation positions and also some orphan sites without a matching C/D snoRNA. Furthermore, immunoprecipitation of Arabidopsis FIB2 identified and demonstrated expression of C/D snoRNAs corresponding to majority of mapped rRNA sites. On the other hand, we show that disruption of Arabidopsis Nucleolin 1 gene (NUC1), encoding a major nucleolar protein, decreases 2'--methylation at specific rRNA sites suggesting functional/structural interconnections of 2'--methylation with nucleolus organization and plant development. Finally, based on our findings and existent database sets, we introduce a new nomenclature system for C/D snoRNA in Arabidopsis plants.

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