Differential Activation of JAK-STAT Signaling Reveals Functional Compartmentalization in Blood Progenitors

Overview

Journal Elife

Specialty Biology

Date 2021 Feb 17

PMID 33594977

Citations 20

Authors

Diana Rodrigues

Yoan Renaud

K VijayRaghavan

Lucas Waltzer

Maneesha S Inamdar

Affiliations

Soon will be listed here.

Abstract

Blood cells arise from diverse pools of stem and progenitor cells. Understanding progenitor heterogeneity is a major challenge. The larval lymph gland is a well-studied model to understand blood progenitor maintenance and recapitulates several aspects of vertebrate hematopoiesis. However in-depth analysis has focused on the anterior lobe progenitors (AP), ignoring the posterior progenitors (PP) from the posterior lobes. Using in situ expression mapping and developmental and transcriptome analysis, we reveal PP heterogeneity and identify molecular-genetic tools to study this abundant progenitor population. Functional analysis shows that PP resist differentiation upon immune challenge, in a JAK-STAT-dependent manner. Upon wasp parasitism, AP downregulate JAK-STAT signaling and form lamellocytes. In contrast, we show that PP activate STAT92E and remain undifferentiated, promoting survival. knockdown or genetically reducing JAK-STAT signaling permits PP lamellocyte differentiation. We discuss how heterogeneity and compartmentalization allow functional segregation in response to systemic cues and could be widely applicable.

Citing Articles

Molecular Mechanisms of Drosophila Hematopoiesis.

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