Insulin-like 3 Affects Zebrafish Spermatogenic Cells Directly and Via Sertoli Cells

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Feb 16

PMID 33589679

Citations 9

Authors

Diego Crespo

Luiz H C Assis

Yu Ting Zhang

Diego Safian

Tomasz Furmanek

Kai Ove Skaftnesmo

Birgitta Norberg

Wei Ge

Yung-Ching Choi

Marjo J den Broeder

Juliette Legler

Jan Bogerd

Rudiger W Schulz

Affiliations

Soon will be listed here.

Abstract

Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl3), a Leydig cell-derived growth factor found in all vertebrates. Little information is available regarding Insl3 function in adult spermatogenesis. The Insl3 receptors Rxfp2a and 2b were expressed by type A spermatogonia and Sertoli and myoid cells, respectively, in zebrafish testis tissue. Loss of insl3 increased germ cell apoptosis in males starting at 9 months of age, but spermatogenesis appeared normal in fully fertile, younger adults. Insl3 changed the expression of 409 testicular genes. Among others, retinoic acid (RA) signaling was up- and peroxisome proliferator-activated receptor gamma (Pparg) signaling was down-regulated. Follow-up studies showed that RA and Pparg signaling mediated Insl3 effects, resulting in the increased production of differentiating spermatogonia. This suggests that Insl3 recruits two locally active nuclear receptor pathways to implement pituitary (Fsh) stimulation of spermatogenesis.

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