Cardioprotective Effects of Erythropoietin in Diabetic Rats Determined by CD34 and Vascular Endothelial Growth Factor Levels

Overview

Journal Arch Med Sci Atheroscler Dis

Date 2021 Feb 15

PMID 33585719

Citations 1

Authors

Yidem Melis Oztas

Mert Meric

Metin Onur Beyaz

Mehmet Akif Onalan

Kivilcim Sonmez

Kerem Oter

Sedat Ziyade

Beyhan Omer

Ufuk Alpagut

Murat Ugurlucan

Affiliations

Soon will be listed here.

Abstract

Introduction: In this study, the effects of diabetes mellitus on the cardiovascular system were investigated by assessing the stem cell levels in serum and heart and compared with the normal population. Additionally, efficacy of erythropoietin, which is known to increase stem cells, was studied in diabetic rats.

Material And Methods: Twenty-five male Sprague Dawley rats were divided into three groups as a control group (group 1), diabetic group (group 2) and erythropoietin induced diabetic group (group 3). A diabetes model was created with streptozocin. In group 3 rats received 3000 U/kg of erythropoietin. At the end of 1 month blood reticulocyte levels, degree of tissue fibrosis and immunohistochemical assessment of reliable stem cell markers, CD34 and vascular endothelial growth factor (VEGF), were analyzed.

Results: The increase in the blood glucose levels resulted in a significant decrease in reticulocyte levels in group 2. The increase in blood glucose levels resulted in a statistically significant increase in tissue level of fibrosis, CD34 and VEGF. When the rats in groups 1 and 2 were compared, the fibrosis, CD34 and VEGF levels were found to increase significantly. When group 2 and group 3 were compared, the amount of fibrosis was lower and the levels of CD34 and VEGF were significantly higher in group 3 than group 2.

Conclusions: The results of our study indicated that the amount of CD34 and VEGF which function in cellular protection and tissue regeneration may be enhanced with safely applicable erythropoietin leading to increase in reticulocyte levels in serum, and CD34 and VEGF levels in right atrium, right ventricle, left atrium, and left ventricle as a protective mechanism in diabetic rats.

Citing Articles

Cardiomyocyte crosstalk with endothelium modulates cardiac structure, function, and ischemia-reperfusion injury susceptibility through erythropoietin.

Marrow J, Alshamali R, Edgett B, Allwood M, Cochrane K, Al-Sabbag S Front Physiol. 2024; 15:1397049.

PMID: 39011088 PMC: 11246973. DOI: 10.3389/fphys.2024.1397049.

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