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Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Based on Illumina Sequencing

Overview
Journal Pol J Microbiol
Specialty Microbiology
Date 2021 Feb 12
PMID 33574857
Citations 3
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Abstract

is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The endophytes in the plant have a significant role in promoting plant growth and enhancing plant stress resistance. However, the endophytic bacterial structure associated with halophyte is still not revealed. In this project, the construction and diversity of endophytic bacterial consortium associated with different tissues of were illustrated with high throughput sequencing of the V3-V4 region of the bacterial 16S rRNA. The results resolved that the diversity and richness of endophytic bacteria were significantly higher in root than in leaf and stem. The operational taxonomic units (OTU) analysis demonstrated that the Actinobacteria and Proteobacteria were dominant in all the samples at the phylum level, and were the dominant genera. Our results unraveled that the bacterial communities differed among different tissues of . Endophytic bacterial communities in leaf and stem shared more similarity than that in the root. Furthermore, the difference of bacteria community and structure among different tissues were also detected by principal coordinate analysis. Taken altogether, we can conclude that the bacterial communities of different tissues are unique, which could facilitate understanding the diversity of endophytic bacteria in .

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