SNPs in the 3'-untranslated Region of Confer Risk of Type 2 Diabetes Mellitus in a South-east Iranian Population: Evidences from Case-control and Bioinformatics Studies

Overview

Journal J Diabetes Metab Disord

Specialty Endocrinology

Date 2021 Feb 8

PMID 33553018

Citations 8

Authors

Saman Sargazi

Milad Heidari Nia

Fariba Mirani Sargazi

Roghayeh Sheervalilou

Ramin Saravani

Shekoufeh Mirinejad

Affiliations

Soon will be listed here.

Abstract

Background: Type 2 diabetes mellitus (T2DM) is a heterogenic disease with increasing incidence. The gene encodes an islet zinc transporter (ZnT8), and its variants have been associated with glucose and pro-insulin levels. This study was aimed to examine the effects of a missense variant (rs13266634 C/T), and two 3'UTR variants (rs2466294 C/G and rs2466293 T/C) in gene on T2DM risk in a south-east Iranian population.

Methods: In this experiment, 450 patients diagnosed with T2DM and 453 healthy subjects from the same geographic area were enrolled. Genotypes were amplified using the ARMS-PCR method. analyses were performed to determine the effects of the variants on the local structure of mRNA, splicing patterns, and potential miRNA-gene interactions as well.

Results: Significant differences were noticed between cases and controls regarding the genotypic and allelic distribution of the studied variants. As regards rs2466293 and rs2466294 variants, enhanced risk of T2DM was found under allelic, dominant, recessive, and codominant models (OR > 1). Besides, different genetic models of rs13266634 were associated with decreased risk of T2DM (OR < 1). Bioinformatics analyses indicated that the rs2466293 variant might influence the binding of some miRNAs, while the G-allele of rs2466294 decreased the stability of

Conclusions: In our population, both SNPs in the 3'-untranslated region of increased the risk of T2DM, while the rs13266634 variant showed a protective association against T2DM susceptibility. Investigating the effects of other variants in this gene or other ZnTs can further indicate such associations in subjects from the same ethnicity.

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