Cigarette Smoke Extract Affects Methylation Status and Attenuates Sca-1 Expression of Mouse Endothelial Progenitor Cell in Vitro

Overview

Journal Tob Induc Dis

Date 2021 Feb 5

PMID 33542680

Citations 2

Authors

Zhi-Hui He

Yan Chen

Ping Chen

Li-Hua Xie

Gui-Bin Liang

Hong-Liang Zhang

Huai-Huai Peng

Affiliations

Soon will be listed here.

Abstract

Introduction: Endothelial dysfunction appears in many smoking-related diseases, it is also an important pathophysiological feature. Endothelial progenitor cells (EPCs) are precursors of endothelial cells and have a crucial effect on the repair and maintenance of endothelial integrity. Sca-1 is not only common in bone marrow-derived hematopoietic stem cells (HSCs), but it is also expressed in nonhematopoietic organs by tissue-resident stem and progenitor cells. The aim of this study is to investigate the impact of cigarette smoke extract (CSE) on the function of bone marrow-derived EPCs and the expression level of Sca-1 in EPCs, and also whether the methylation of Sca-1 is involved in EPC dysfunction.

Methods: We measured EPC capacities including adhesion, secretion and proliferation, the concentration of endothelial nitric oxide synthase (eNOS) and apoptosis-inducing factor (AIF) in cell culture supernatant, and also Sca-1 expression and promoter methylation in EPCs induced by CSE. Decitabine (Dec) was applied to test whether it could alter the impact caused by CSE.

Results: The adhesion, proliferation and secretion ability of EPCs can be induced to be decreased by CSE , accompanied by decreased concentrations of AIF and eNOS in cell culture supernatant and decreased Sca-1 expression in EPCs. In addition, Dec could partly attenuate the impact described above. There were no significant differences in the quantitative analysis of Sca-1 promoter methylation among different groups.

Conclusions: The decreased Sca-1 expression was related to EPC dysfunction induced by CSE. EPC dysfunction resulting from CSE may be related to methylation mechanism, but not the methylation of Sca-1 promoter.

Citing Articles

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