Perfusion, Cryopreservation, and Nanowarming of Whole Hearts Using Colloidally Stable Magnetic Cryopreservation Agent Solutions

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2021 Feb 1

PMID 33523997

Citations 32

Authors

Andreina Chiu-Lam

Edward Staples

Carl J Pepine

Carlos Rinaldi

Affiliations

Soon will be listed here.

Abstract

Nanowarming of cryopreserved organs perfused with magnetic cryopreservation agents (mCPAs) could increase donor organ utilization by extending preservation time and avoiding damage caused by slow and nonuniform rewarming. Here, we report formulation of an mCPA containing superparamagnetic iron oxide nanoparticles (SPIONs) that are stable against aggregation in the cryopreservation agent VS55 before and after vitrification and nanowarming and that achieve high-temperature rise rates of up to 321°C/min under an alternating magnetic field. These SPIONs and mCPAs have low cytotoxicity against primary cardiomyocytes. We demonstrate successful perfusion of whole rat hearts with the mCPA and removal using Custodiol HTK solution, even after vitrification, cryostorage in liquid nitrogen for 1 week, and nanowarming under an alternating magnetic field. Quantification of SPIONs in the hearts using magnetic particle imaging demonstrates that the formulated mCPAs are suitable for perfusion, vitrification, and nanowarming of whole organs with minimal residual iron in tissues.

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