Structures of RC-LH1 Complexes with Open or Closed Quinone Channels

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2021 Feb 1

PMID 33523887

Citations 25

Authors

David J K Swainsbury

Pu Qian

Philip J Jackson

Kaitlyn M Faries

Dariusz M Niedzwiedzki

Elizabeth C Martin

David A Farmer

Lorna A Malone

Rebecca F Thompson

Neil A Ranson

Daniel P Canniffe

Mark J Dickman

Dewey Holten

Christine Kirmaier

Andrew Hitchcock

C Neil Hunter

Affiliations

Soon will be listed here.

Abstract

The reaction-center light-harvesting complex 1 (RC-LH1) is the core photosynthetic component in purple phototrophic bacteria. We present two cryo-electron microscopy structures of RC-LH1 complexes from A 2.65-Å resolution structure of the RC-LH1-W complex consists of an open 14-subunit LH1 ring surrounding the RC interrupted by protein-W, whereas the complex without protein-W at 2.80-Å resolution comprises an RC completely encircled by a closed, 16-subunit LH1 ring. Comparison of these structures provides insights into quinone dynamics within RC-LH1 complexes, including a previously unidentified conformational change upon quinone binding at the RC Q site, and the locations of accessory quinone binding sites that aid their delivery to the RC. The structurally unique protein-W prevents LH1 ring closure, creating a channel for accelerated quinone/quinol exchange.

Citing Articles

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