Structure of Tumour Necrosis Factor by X-ray Solution Scattering and Preliminary Studies by Single Crystal X-ray Diffraction

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 1988 Jan 20

PMID 3351931

Citations 8

Authors

R Fourme

R Kahn

T Prange

P Vachette

J Tavernier

G Hauquier

W Niers

Affiliations

Soon will be listed here.

Abstract

The structure of tumour necrosis factor has been investigated by X-ray small-angle scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is 25.5 A. A plausible model accounting for the scattering curves consists of an elongated trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 A. Tumour necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a single trimer in the asymmetric unit. The diffraction extends to 3.5 A.

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