Study on the Interactive Effect of Deoxynivalenol and Clostridium Perfringens on the Jejunal Health of Broiler Chickens

Overview

Journal Poult Sci

Publisher Elsevier

Specialty Veterinary Medicine

Date 2021 Feb 1

PMID 33518310

Citations 9

Authors

Fangshen Guo

Fangyuan Wang

Haiyan Ma

Zhouzheng Ren

Xiaojun Yang

Xin Yang

Affiliations

Soon will be listed here.

Abstract

With global warming and ban on antibiotics, it occurs occasionally that deoxynivalenol (DON) together with Clostridium perfringens impairs the gut health of broiler chickens. However, the interactive effect of DON and C. perfringens on intestinal health is still unknown. A total of 120 one-day-old Arbor Acres broilers were randomly distributed to 4 groups. Birds were gavaged with C. perfringens (8 × 10 CFU/d per bird) or sterile medium and fed a DON diet (0 or 5 mg of DON per kg diet) to investigate the interactive effects. The main effect analysis showed that DON diet significantly downregulated (P < 0.05) the mRNA expression of mucin-2, B-cell lymphoma-2-associated X, and cysteinyl aspartate-specific proteinase-3 of jejunal mucosa; decreased (P < 0.05) the indexes of ACE, Chao1, Shannon, and Simpson; and also decreased the relative abundance of the phylum Bacteroidete and the genera Lactococcus in jejunal contents of broilers chickens. Meanwhile, C. perfringens significantly increased (P < 0.05) crypt depth; decreased (P < 0.05) the ratio of villi height to crypt depth, the activity of jejunal diamine oxidase, and the relative abundance of Lactococcus; and upregulated (P < 0.05) the relative expression of B-cell lymphoma-2 and cysteinyl aspartate-specific proteinase-8. Furthermore, the interactions between DON and C. perfringens were most significant (P < 0.05) in the mRNA expression of lipopolysaccharide-induced TNF factor (LITAF) and TLR-4, the abundance of the genera Lactococcus in jejunal contents, and butyric acid concentrations in cecal contents of birds. Finally, Spearman correlation analysis suggested that the most negative correlations (P < 0.05) with the abundance of the genera except Lactobacillus were observed within the mRNA expression of LITAF. The abundance of Lactococcus had a positive correlation (P < 0.05) with the expression of Caspase-3. Most genera except Lactobacillus negatively correlated (P < 0.05) with acetic acid, butyric acid, and total short-chain fatty acids. In conclusion, dietary deoxynivalenol and C. perfringens challenge had a harmful effect on the jejunal health and should be carefully monitored in broiler production.

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References

Osselaere A, Santos R, Hautekiet V, De Backer P, Chiers K, Ducatelle R . Deoxynivalenol impairs hepatic and intestinal gene expression of selected oxidative stress, tight junction and inflammation proteins in broiler chickens, but addition of an adsorbing agent shifts the effects to the distal parts of the small intestine. PLoS One. 2013; 8(7):e69014. PMC: 3724867. DOI: 10.1371/journal.pone.0069014. View

Barbieri F, Montanari C, Gardini F, Tabanelli G . Biogenic Amine Production by Lactic Acid Bacteria: A Review. Foods. 2019; 8(1). PMC: 6351943. DOI: 10.3390/foods8010017. View

Letai A, Bassik M, Walensky L, Sorcinelli M, Weiler S, Korsmeyer S . Distinct BH3 domains either sensitize or activate mitochondrial apoptosis, serving as prototype cancer therapeutics. Cancer Cell. 2002; 2(3):183-92. DOI: 10.1016/s1535-6108(02)00127-7. View

Yang X, Li L, Duan Y, Yang X . Antioxidant activity of JM113 in vitro and its protective effect on broiler chickens challenged with deoxynivalenol. J Anim Sci. 2017; 95(2):837-846. DOI: 10.2527/jas.2016.0789. View

Sattler S . The Role of the Immune System Beyond the Fight Against Infection. Adv Exp Med Biol. 2017; 1003:3-14. DOI: 10.1007/978-3-319-57613-8_1. View

Robert H, Payros D, Pinton P, Theodorou V, Mercier-Bonin M, Oswald I . Impact of mycotoxins on the intestine: are mucus and microbiota new targets?. J Toxicol Environ Health B Crit Rev. 2017; 20(5):249-275. DOI: 10.1080/10937404.2017.1326071. View

Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli K . Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998; 17(6):1675-87. PMC: 1170515. DOI: 10.1093/emboj/17.6.1675. View

Girardet C, Bonnet M, Jdir R, Sadoud M, Thirion S, Tardivel C . The food-contaminant deoxynivalenol modifies eating by targeting anorexigenic neurocircuitry. PLoS One. 2011; 6(10):e26134. PMC: 3192137. DOI: 10.1371/journal.pone.0026134. View

Awad W, Ruhnau D, Hess C, Doupovec B, Schatzmayr D, Hess M . Feeding of deoxynivalenol increases the intestinal paracellular permeability of broiler chickens. Arch Toxicol. 2019; 93(7):2057-2064. DOI: 10.1007/s00204-019-02460-3. View

10.

Uzal F, Freedman J, Shrestha A, Theoret J, Garcia J, Awad M . Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease. Future Microbiol. 2014; 9(3):361-77. PMC: 4155746. DOI: 10.2217/fmb.13.168. View

11.

Cogliati S, Frezza C, Soriano M, Varanita T, Quintana-Cabrera R, Corrado M . Mitochondrial cristae shape determines respiratory chain supercomplexes assembly and respiratory efficiency. Cell. 2013; 155(1):160-71. PMC: 3790458. DOI: 10.1016/j.cell.2013.08.032. View

12.

Pinton P, Graziani F, Pujol A, Nicoletti C, Paris O, Ernouf P . Deoxynivalenol inhibits the expression by goblet cells of intestinal mucins through a PKR and MAP kinase dependent repression of the resistin-like molecule β. Mol Nutr Food Res. 2015; 59(6):1076-87. DOI: 10.1002/mnfr.201500005. View

13.

Streit E, Naehrer K, Rodrigues I, Schatzmayr G . Mycotoxin occurrence in feed and feed raw materials worldwide: long-term analysis with special focus on Europe and Asia. J Sci Food Agric. 2013; 93(12):2892-9. DOI: 10.1002/jsfa.6225. View

14.

Zhou H, Lepp D, Pei Y, Liu M, Yin X, Ma R . Influence of pCP1NetB ancillary genes on the virulence of poultry necrotic enteritis strain CP1. Gut Pathog. 2017; 9:6. PMC: 5251324. DOI: 10.1186/s13099-016-0152-y. View

15.

Vandenbroucke V, Croubels S, Martel A, Verbrugghe E, Goossens J, Van Deun K . The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops. PLoS One. 2011; 6(8):e23871. PMC: 3166085. DOI: 10.1371/journal.pone.0023871. View

16.

Dalile B, Van Oudenhove L, Vervliet B, Verbeke K . The role of short-chain fatty acids in microbiota-gut-brain communication. Nat Rev Gastroenterol Hepatol. 2019; 16(8):461-478. DOI: 10.1038/s41575-019-0157-3. View

17.

Xue G, Barekatain R, Wu S, Choct M, Swick R . Dietary L-glutamine supplementation improves growth performance, gut morphology, and serum biochemical indices of broiler chickens during necrotic enteritis challenge. Poult Sci. 2018; 97(4):1334-1341. DOI: 10.3382/ps/pex444. View

18.

Li F, Wang J, Huang L, Chen H, Wang C . Effects of Adding Clostridium sp. WJ06 on Intestinal Morphology and Microbial Diversity of Growing Pigs Fed with Natural Deoxynivalenol Contaminated Wheat. Toxins (Basel). 2017; 9(12). PMC: 5744103. DOI: 10.3390/toxins9120383. View

19.

Liu S, Zhang D, Liu Y, Zhou D, Yang H, Zhang K . Circular RNA circ_0001105 protects the intestinal barrier of septic rats by inhibiting inflammation and oxidative damage and YAP1 expression. Gene. 2020; 755:144897. DOI: 10.1016/j.gene.2020.144897. View

20.

Rodrigues I, Naehrer K . A three-year survey on the worldwide occurrence of mycotoxins in feedstuffs and feed. Toxins (Basel). 2012; 4(9):663-75. PMC: 3475222. DOI: 10.3390/toxins4090663. View