Effects of Vitrification on the Blastocyst Gene Expression Profile in a Porcine Model

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Jan 30

PMID 33513717

Citations 11

Authors

Cristina Cuello

Cristina A Martinez

Josep M Cambra

Inmaculada Parrilla

Heriberto Rodriguez-Martinez

Maria A Gil

Emilio A Martinez

Affiliations

Soon will be listed here.

Abstract

This study was designed to investigate the impact of vitrification on the transcriptome profile of blastocysts using a porcine () model and a microarray approach. Blastocysts were collected from weaned sows ( = 13). A total of 60 blastocysts were vitrified (treatment group). After warming, vitrified embryos were cultured in vitro for 24 h. Non-vitrified blastocysts ( = 40) were used as controls. After the in vitro culture period, the embryo viability was morphologically assessed. A total of 30 viable embryos per group (three pools of 10 from 4 different donors each) were subjected to gene expression analysis. A fold change cut-off of ±1.5 and a restrictive threshold at -value < 0.05 were used to distinguish differentially expressed genes (DEGs). The survival rates of vitrified/warmed blastocysts were similar to those of the control (nearly 100%, n.s.). A total of 205 (112 upregulated and 93 downregulated) were identified in the vitrified blastocysts compared to the control group. The vitrification/warming impact was moderate, and it was mainly related to the pathways of cell cycle, cellular senescence, gap junction, and signaling for TFGβ, p53, Fox, and MAPK. In conclusion, vitrification modified the transcriptome of in vivo-derived porcine blastocysts, resulting in minor gene expression changes.

Citing Articles

Transcriptomic Analysis of Vitrified-Warmed vs. Fresh Mouse Blastocysts: Cryo-Induced Physiological Mechanisms and Implantation Impact.

Lee C, Tsai H, Cheng E, Lee T, Lin P, Lee M Int J Mol Sci. 2024; 25(16).

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Safety of embryo cryopreservation: insights from mid-term placental transcriptional changes.

Luo Q, Zhang S, Wu H, Mo J, Yu J, He R Reprod Biol Endocrinol. 2024; 22(1):80.

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Long-term health risk of offspring born from assisted reproductive technologies.

Zhang S, Luo Q, Meng R, Yan J, Wu Y, Huang H J Assist Reprod Genet. 2023; 41(3):527-550.

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Melatonin improves the vitrification of sheep morulae by modulating transcriptome.

Ji P, Liu Y, Yan L, Jia Y, Zhao M, Lv D Front Vet Sci. 2023; 10:1212047.

PMID: 37920328 PMC: 10619913. DOI: 10.3389/fvets.2023.1212047.

The Use of a Brief Synchronization Treatment after Weaning, Combined with Superovulation, Has Moderate Effects on the Gene Expression of Surviving Pig Blastocysts.

Gonzalez-Ramiro H, Gil M, Cuello C, Cambra J, Gonzalez-Plaza A, Vazquez J Animals (Basel). 2023; 13(9).

PMID: 37174605 PMC: 10177444. DOI: 10.3390/ani13091568.

References

Bindea G, Mlecnik B, Hackl H, Charoentong P, Tosolini M, Kirilovsky A . ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. Bioinformatics. 2009; 25(8):1091-3. PMC: 2666812. DOI: 10.1093/bioinformatics/btp101. View

Mantione K, Kream R, Kuzelova H, Ptacek R, Raboch J, Samuel J . Comparing bioinformatic gene expression profiling methods: microarray and RNA-Seq. Med Sci Monit Basic Res. 2014; 20:138-42. PMC: 4152252. DOI: 10.12659/MSMBR.892101. View

Martinez E, Martinez C, Nohalez A, Sanchez-Osorio J, Vazquez J, Roca J . Nonsurgical deep uterine transfer of vitrified, in vivo-derived, porcine embryos is as effective as the default surgical approach. Sci Rep. 2015; 5:10587. PMC: 4450750. DOI: 10.1038/srep10587. View

Gambino V, de Michele G, Venezia O, Migliaccio P, DallOlio V, Bernard L . Oxidative stress activates a specific p53 transcriptional response that regulates cellular senescence and aging. Aging Cell. 2013; 12(3):435-45. PMC: 3709138. DOI: 10.1111/acel.12060. View

Tran S, Wang L, Le J, Guan J, Wu L, Zou J . Altered methylation of the DNA repair gene MGMT is associated with neural tube defects. J Mol Neurosci. 2011; 47(1):42-51. DOI: 10.1007/s12031-011-9676-2. View

Lee W, Jang S, Lee S, Park J, Jeon R, Subbarao R . Selection of reference genes for quantitative real-time polymerase chain reaction in porcine embryos. Reprod Fertil Dev. 2015; 29(2):357-367. DOI: 10.1071/RD14393. View

Kader A, Agarwal A, Abdelrazik H, Sharma R, Ahmady A, Falcone T . Evaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing. Fertil Steril. 2008; 91(5 Suppl):2087-94. DOI: 10.1016/j.fertnstert.2008.04.049. View

Mullen A, Wrana J . TGF-β Family Signaling in Embryonic and Somatic Stem-Cell Renewal and Differentiation. Cold Spring Harb Perspect Biol. 2017; 9(7). PMC: 5495062. DOI: 10.1101/cshperspect.a022186. View

Perleberg C, Kind A, Schnieke A . Genetically engineered pigs as models for human disease. Dis Model Mech. 2018; 11(1). PMC: 5818075. DOI: 10.1242/dmm.030783. View

10.

Vousden K, Lu X . Live or let die: the cell's response to p53. Nat Rev Cancer. 2002; 2(8):594-604. DOI: 10.1038/nrc864. View

11.

Chen Y, Dai J, Wu C, Zhang S, Sun L, Zhang D . Apoptosis and developmental capacity of vitrified parthenogenetic pig blastocysts. Anim Reprod Sci. 2018; 198:137-144. DOI: 10.1016/j.anireprosci.2018.09.012. View

12.

Cuello C, Berthelot F, Delaleu B, Venturi E, Pastor L, Vazquez J . The effectiveness of the stereomicroscopic evaluation of embryo quality in vitrified-warmed porcine blastocysts: an ultrastructural and cell death study. Theriogenology. 2007; 67(5):970-82. DOI: 10.1016/j.theriogenology.2006.11.011. View

13.

Gupta A, Singh J, Dufort I, Robert C, Dias F, Anzar M . Transcriptomic difference in bovine blastocysts following vitrification and slow freezing at morula stage. PLoS One. 2017; 12(11):e0187268. PMC: 5667772. DOI: 10.1371/journal.pone.0187268. View

14.

Adamson G, de Mouzon J, Chambers G, Zegers-Hochschild F, Mansour R, Ishihara O . International Committee for Monitoring Assisted Reproductive Technology: world report on assisted reproductive technology, 2011. Fertil Steril. 2018; 110(6):1067-1080. DOI: 10.1016/j.fertnstert.2018.06.039. View

15.

Cuello C, Martinez C, Nohalez A, Parrilla I, Roca J, Gil M . Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium. Sci Rep. 2016; 6:33915. PMC: 5036199. DOI: 10.1038/srep33915. View

16.

Castillo-Martin M, Yeste M, Pericuesta E, Morato R, Gutierrez-Adan A, Bonet S . Effects of vitrification on the expression of pluripotency, apoptotic and stress genes in in vitro-produced porcine blastocysts. Reprod Fertil Dev. 2014; 27(7):1072-81. DOI: 10.1071/RD13405. View

17.

Kruiswijk F, Labuschagne C, Vousden K . p53 in survival, death and metabolic health: a lifeguard with a licence to kill. Nat Rev Mol Cell Biol. 2015; 16(7):393-405. DOI: 10.1038/nrm4007. View

18.

Stelzer G, Rosen N, Plaschkes I, Zimmerman S, Twik M, Fishilevich S . The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses. Curr Protoc Bioinformatics. 2016; 54:1.30.1-1.30.33. DOI: 10.1002/cpbi.5. View

19.

Aktoprakligil Aksu D, Agca C, Aksu S, Bagis H, Akkoc T, Caputcu A . Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts. Mol Reprod Dev. 2012; 79(9):613-25. DOI: 10.1002/mrd.22068. View

20.

Jaroudi S, SenGupta S . DNA repair in mammalian embryos. Mutat Res. 2006; 635(1):53-77. DOI: 10.1016/j.mrrev.2006.09.002. View