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Genome Sequences of 10 SARS-CoV-2 Viral Strains Obtained by Nanopore Sequencing of Nasopharyngeal Swabs in Malta

Overview
Journal Microbiol Resour Announc
Publisher American Society for Microbiology
Specialty Microbiology
Date 2021 Jan 29
PMID 33509993
Citations 2
Authors
Manuele Biazzo
Silvia Madeddu
Elfath Elnifro
Tessabella Sultana
Josie Muscat
Christian A Scerri
Francesco Santoro
David Pinzauti
Affiliations
Soon will be listed here.
Abstract

The genome sequences of 10 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains from Sliema, Malta, were obtained by Nanopore sequencing using the amplicon sequencing approach developed by the Artic Network. The assembled genomes were analyzed with Pangolin software and assigned to the B.1 lineage, which is widely circulating in Europe.

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Nanopore sequencing technology and its applications.

Zheng P, Zhou C, Ding Y, Liu B, Lu L, Zhu F MedComm (2020). 2023; 4(4):e316.

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References
1.
Katoh K, Misawa K, Kuma K, Miyata T . MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 2002; 30(14):3059-66. PMC: 135756. DOI: 10.1093/nar/gkf436. View
2.
Letunic I, Bork P . Interactive Tree Of Life (iTOL) v4: recent updates and new developments. Nucleic Acids Res. 2019; 47(W1):W256-W259. PMC: 6602468. DOI: 10.1093/nar/gkz239. View
3.
Milne I, Stephen G, Bayer M, Cock P, Pritchard L, Cardle L . Using Tablet for visual exploration of second-generation sequencing data. Brief Bioinform. 2012; 14(2):193-202. DOI: 10.1093/bib/bbs012. View
4.
Meredith L, Hamilton W, Warne B, Houldcroft C, Hosmillo M, Jahun A . Rapid implementation of SARS-CoV-2 sequencing to investigate cases of health-care associated COVID-19: a prospective genomic surveillance study. Lancet Infect Dis. 2020; 20(11):1263-1272. PMC: 7806511. DOI: 10.1016/S1473-3099(20)30562-4. View
5.
Rambaut A, Holmes E, OToole A, Hill V, McCrone J, Ruis C . A dynamic nomenclature proposal for SARS-CoV-2 lineages to assist genomic epidemiology. Nat Microbiol. 2020; 5(11):1403-1407. PMC: 7610519. DOI: 10.1038/s41564-020-0770-5. View