[Overexpression of LncRNA MEG3 Inhibits Proliferation and Invasion of Glioblastoma U251 Cells by Suppressing HIF1 Expression]

Overview

Journal Nan Fang Yi Ke Da Xue Xue Bao

Specialty General Medicine

Date 2021 Jan 29

PMID 33509767

Citations 2

Authors

Qizhi Luo

Fan Zhang

Wei Li

Fang Wang

Lixiang Wu

Baisheng Huang

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the effects of overexpression of long noncoding RNA (lncRNA) MEG3 on the proliferation and invasion of glioblastoma U251 cells by suppressing the expression of hypoxia inducible factor 1(HIF1).

Methods: The expression of lncRNA MEG3 and HIF1 mRNA were examined in human fetal glial cells (HFGCs) and U251 cells using realtime quantitative PCR (qRT-PCR), and the expression of HIF1 protein was detected with Western blotting.U251 cells in normal culture or transfected with pcDNA3.1 vector (NC group) or pcDNA3.1-MEG3 vector lipofectamine2000 were exposed to hypoxia for 12h, and the expressions of HIF1 mRNA and protein were detected with qRT-PCR and Western blotting, respectively.MTT assay and Transwell assay were employed to examine the influence of MEG3 overexpression on the proliferation and invasion of U251 cells.

Results: The expression of MEG3 was significantly lower and HIF1 mRNA and protein expressions were significantly higher in U251 cells than in HFGCs ( < 0.05).In U251 cells, overexpression of MEG3 significantly decreased the mRNA and protein expressions of HIF1( < 0.05).Hypoxic exposure for 12h also resulted in significantly lowered expression of HIF1 protein in U251 cells ( < 0.05).Overexpression of MEG3 obviously suppressed the proliferation and invasiveness of U251 cells ( < 0.05).

Conclusions: MEG3 overexpression inhibits the proliferation and invasion of U251 cells through suppressing the expression of HIF1 mRNA and protein, suggesting that MEG3 may serve as a potential therapeutic target for glioblastomas.

Citing Articles

[Overexpression of LncRNA MEG3 promotes ferroptosis and enhances chemotherapy sensitivity of hepatocellular carcinoma cells to cisplatin].

Zhu Q, Huang B, Wei L, Luo Q Nan Fang Yi Ke Da Xue Xue Bao. 2024; 44(1):17-24.

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Insights into the Role of LncRNAs and miRNAs in Glioma Progression and Their Potential as Novel Therapeutic Targets.

Kciuk M, Yahya E, Mohamed M, Abdulsamad M, Allaq A, Gielecinska A Cancers (Basel). 2023; 15(13).

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