Combined Negative Dielectrophoresis with a Flexible SERS Platform As a Novel Strategy for Rapid Detection and Identification of Bacteria

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2021 Jan 28

PMID 33507352

Citations 7

Authors

Ariadna B Nowicka

Marta Czaplicka

Tomasz Szymborski

Agnieszka Kaminska

Affiliations

Soon will be listed here.

Abstract

Surface-enhanced Raman spectroscopy (SERS) is a vibrational method successfully applied in analytical chemistry, molecular biology and medical diagnostics. In this article, we demonstrate the combination of the negative dielectrophoretic (nDEP) phenomenon and a flexible surface-enhanced Raman platform for quick isolation (3 min), concentration and label-free identification of bacteria. The platform ensures a strong enhancement factor, high stability and reproducibility for the SERS response of analyzed samples. By introducing radial dielectrophoretic forces directed at the SERS platform, we can efficiently execute bacterial cell separation, concentration and deposition onto the SERS-active surface, which simultaneously works as a counter electrode and thus enables such hybrid DEP-SERS device vibration-based detection. Additionally, we show the ability of our DEP-SERS system to perform rapid, cultivation-free, direct detection of bacteria in urine and apple juice samples. The device provides new opportunities for the detection of pathogens.

Citing Articles

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