Expression of Vitamin D 1α-hydroxylase in Human Gingival Fibroblasts in Vivo

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2021 Jan 28

PMID 33505780

Citations 2

Authors

Kaining Liu

Bing Han

Jianxia Hou

Jianyun Zhang

Jing Su

Huanxin Meng

Affiliations

Soon will be listed here.

Abstract

Background: Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo.

Methods: Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals' gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR.

Results: Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group.

Conclusions: Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.

Citing Articles

Association of Serum Vitamin D With Periodontal Disease.

Li Y, Wang J, Cai Y, Chen H Int Dent J. 2023; 73(5):777-783.

PMID: 37419778 PMC: 10509416. DOI: 10.1016/j.identj.2023.06.004.

Association between Vitamin D Receptor Gene Polymorphisms and Periodontal Bacteria: A Clinical Pilot Study.

Cafiero C, Grippaudo C, DellAquila M, Cimmino P, DAddona A, De Angelis P Biomolecules. 2022; 12(6).

PMID: 35740958 PMC: 9221517. DOI: 10.3390/biom12060833.

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