Vertical Sleeve Gastrectomy Induces Distinctive Transcriptomic Responses in Liver, Fat and Muscle

Overview

Journal Sci Rep

Specialty Science

Date 2021 Jan 28

PMID 33504853

Citations 8

Authors

Chang Ho Ahn

Eun Hye Choi

Hyunjung Lee

Woochan Lee

Jong-Il Kim

Young Min Cho

Affiliations

Soon will be listed here.

Abstract

Vertical sleeve gastrectomy (VSG) is the most commonly performed bariatric/metabolic surgery, exhibiting a high rate of diabetes remission in humans. To elucidate the molecular mechanisms of VSG, we performed transcriptomic analysis of the liver, fat, and muscle in VSG mice. C57BL/6 mice fed a high-fat diet were randomly assigned to sham or VSG surgery. The sham-operated mice were fed ad libitum (sham group) or pair-fed (sham-PF group) matching their food intake to the VSG-operated mice. Comparative transcriptomic analysis of the liver, fat, and muscle using RNA sequencing was performed. VSG reduced body weight and improved glucose tolerance compared to the sham group, but not more than the sham-PF group. Improvement in fatty liver and adipose tissue inflammation was comparable between VSG and sham-PF. However, global gene expression profiles showed distinctive changes in the liver, fat, and muscle of the VSG group compared to both the sham or sham-PF groups. The liver showed the most prominent gene expression changes. Immune response-related pathways were commonly upregulated in the three organs of the VSG group compared to the sham or sham-PF. VSG induces organ-specific gene expression changes in the liver, fat, and muscle, which may play critical roles in metabolic improvements after VSG.

Citing Articles

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Sleeve gastrectomy causes weight-loss independent improvements in hepatic steatosis.

McGlone E, Siebert M, Dore M, Hope D, Davies I, Owen B Liver Int. 2023; 43(9):1890-1900.

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