Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells Upon SARS-CoV-2 Infection

Overview

Journal Biomol Ther (Seoul)

Specialty Pharmacology

Date 2021 Jan 28

PMID 33504682

Citations 27

Authors

Byoung Kwon Park

Dongbum Kim

Sangkyu Park

Sony Maharjan

JinSoo Kim

Jun-Kyu Choi

Madhav Akauliya

Younghee Lee

Hyung-Joo Kwon

Affiliations

Soon will be listed here.

Abstract

Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is responsible for the current coronavirus disease 2019 (COVID-19) pandemic. Signaling pathways that are essential for virus production have potential as therapeutic targets against COVID-19. In this study, we investigated cellular responses in two cell lines, Vero and Calu-3, upon SARS-CoV-2 infection and evaluated the effects of pathway-specific inhibitors on virus production. SARS-CoV-2 infection induced dephosphorylation of STAT1 and STAT3, high virus production, and apoptosis in Vero cells. However, in Calu-3 cells, SARS-CoV-2 infection induced long-lasting phosphorylation of STAT1 and STAT3, low virus production, and no prominent apoptosis. Inhibitors that target STAT3 phosphorylation and dimerization reduced SARS-CoV-2 production in Calu-3 cells, but not in Vero cells. These results suggest a necessity to evaluate cellular consequences upon SARS-CoV-2 infection using various model cell lines to find out more appropriate cells recapitulating relevant responses to SARS-CoV-2 infection .

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