Application of Autologous Peripheral Blood Mononuclear Cells into the Area of Spinal Cord Injury in a Subacute Period: A Feasibility Study in Pigs

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2021 Jan 27

PMID 33498942

Citations 3

Authors

Iliya Shulman

Sergei Ogurcov

Alexander Kostennikov

Alexander Rogozin

Ekaterina Garanina

Galina Masgutova

Mikhail Sergeev

Albert Rizvanov

Yana Mukhamedshina

Affiliations

Soon will be listed here.

Abstract

Peripheral blood presents an available source of cells for both fundamental research and clinical use. In our study, we have evaluated the therapeutic potential of peripheral blood mononuclear cells (PBMCs) excluding the preliminary sorting or mobilization of peripheral blood stem cells. We have evaluated the regenerative potential of PBMCs embedded into a fibrin matrix (FM) in a model of pig spinal cord injury. The distribution of transplanted PBMCs in the injured spinal cord was evaluated; PBMCs were shown to penetrate into the deep layers of the spinal cord and concentrate mainly in the grey matter. The results of the current study revealed an increase in the tissue integrity in the area adjacent to the epicenter of injury and the partially restored conduction along posterior columns of the spinal cord in animals after FM+PBMC application. The multiplex analysis of blood serum and cerebrospinal fluid showed the cytokine imbalance to occur without significantly shifting toward pro-inflammatory or anti-inflammatory cytokine cascades.

Citing Articles

Intrathecal Injection of Autologous Mesenchymal Stem-Cell-Derived Extracellular Vesicles in Spinal Cord Injury: A Feasibility Study in Pigs.

Shulman I, Ageeva T, Kostennikov A, Ogurcov S, Tazetdinova L, Kabdesh I Int J Mol Sci. 2023; 24(9).

PMID: 37175946 PMC: 10179045. DOI: 10.3390/ijms24098240.

The Application of Biomaterials in Spinal Cord Injury.

Feng C, Deng L, Yong Y, Wu J, Qin D, Yu L Int J Mol Sci. 2023; 24(1).

PMID: 36614259 PMC: 9821025. DOI: 10.3390/ijms24010816.

Porcine Model of Spinal Cord Injury: A Systematic Review.

Weber-Levine C, Hersh A, Jiang K, Routkevitch D, Tsehay Y, Perdomo-Pantoja A Neurotrauma Rep. 2022; 3(1):352-368.

PMID: 36204385 PMC: 9531891. DOI: 10.1089/neur.2022.0038.

References

Takahashi H, Koda M, Hashimoto M, Furuya T, Sakuma T, Kato K . Transplanted Peripheral Blood Stem Cells Mobilized by Granulocyte Colony-Stimulating Factor Promoted Hindlimb Functional Recovery After Spinal Cord Injury in Mice. Cell Transplant. 2015; 25(2):283-92. DOI: 10.3727/096368915X688146. View

Park J, Lim E, Back S, Na H, Park Y, Sun K . Nerve regeneration following spinal cord injury using matrix metalloproteinase-sensitive, hyaluronic acid-based biomimetic hydrogel scaffold containing brain-derived neurotrophic factor. J Biomed Mater Res A. 2009; 93(3):1091-9. DOI: 10.1002/jbm.a.32519. View

Mukhamedshina Y, Shulman I, Ogurcov S, Kostennikov A, Zakirova E, Akhmetzyanova E . Mesenchymal Stem Cell Therapy for Spinal Cord Contusion: A Comparative Study on Small and Large Animal Models. Biomolecules. 2019; 9(12). PMC: 6995633. DOI: 10.3390/biom9120811. View

Mothe A, Tator C . Advances in stem cell therapy for spinal cord injury. J Clin Invest. 2012; 122(11):3824-34. PMC: 3484454. DOI: 10.1172/JCI64124. View

Itosaka H, Kuroda S, Shichinohe H, Yasuda H, Yano S, Kamei S . Fibrin matrix provides a suitable scaffold for bone marrow stromal cells transplanted into injured spinal cord: a novel material for CNS tissue engineering. Neuropathology. 2008; 29(3):248-57. DOI: 10.1111/j.1440-1789.2008.00971.x. View

Chen C, Foo N, Liu W, Chen S . Infusion of human umbilical cord blood cells ameliorates hind limb dysfunction in experimental spinal cord injury through anti-inflammatory, vasculogenic and neurotrophic mechanisms. Pediatr Neonatol. 2008; 49(3):77-83. DOI: 10.1016/S1875-9572(08)60017-0. View

Gamez Sazo R, Maenaka K, Gu W, Wood P, Bunge M . Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia. Biomaterials. 2012; 33(33):8529-39. PMC: 3521512. DOI: 10.1016/j.biomaterials.2012.07.028. View

Sasaki H, Ishikawa M, Tanaka N, Nakanishi K, Kamei N, Asahara T . Administration of human peripheral blood-derived CD133+ cells accelerates functional recovery in a rat spinal cord injury model. Spine (Phila Pa 1976). 2009; 34(3):249-54. DOI: 10.1097/BRS.0b013e3181913cde. View

Vismara I, Papa S, Rossi F, Forloni G, Veglianese P . Current Options for Cell Therapy in Spinal Cord Injury. Trends Mol Med. 2017; 23(9):831-849. DOI: 10.1016/j.molmed.2017.07.005. View

10.

Fu Q, Liu Y, Liu X, Zhang Q, Chen L, Peng J . Engrafted peripheral blood-derived mesenchymal stem cells promote locomotive recovery in adult rats after spinal cord injury. Am J Transl Res. 2017; 9(9):3950-3966. PMC: 5622241. View

11.

Zhang M, Huang B . The multi-differentiation potential of peripheral blood mononuclear cells. Stem Cell Res Ther. 2012; 3(6):48. PMC: 3580478. DOI: 10.1186/scrt139. View

12.

Kijima Y, Ishikawa M, Sunagawa T, Nakanishi K, Kamei N, Yamada K . Regeneration of peripheral nerve after transplantation of CD133+ cells derived from human peripheral blood. J Neurosurg. 2008; 110(4):758-67. DOI: 10.3171/2008.3.17571. View

13.

Mukhamedshina Y, Akhmetzyanova E, Martynova E, Khaiboullina S, Galieva L, Rizvanov A . Systemic and Local Cytokine Profile following Spinal Cord Injury in Rats: A Multiplex Analysis. Front Neurol. 2017; 8:581. PMC: 5671564. DOI: 10.3389/fneur.2017.00581. View

14.

Haider T, Hoftberger R, Ruger B, Mildner M, Blumer R, Mitterbauer A . The secretome of apoptotic human peripheral blood mononuclear cells attenuates secondary damage following spinal cord injury in rats. Exp Neurol. 2015; 267:230-42. DOI: 10.1016/j.expneurol.2015.03.013. View

15.

Lee J, Jones C, Okon E, Anderson L, Tigchelaar S, Kooner P . A novel porcine model of traumatic thoracic spinal cord injury. J Neurotrauma. 2013; 30(3):142-59. DOI: 10.1089/neu.2012.2386. View

16.

Zhou X, Zoller T, Krieglstein K, Spittau B . TGFβ1 inhibits IFNγ-mediated microglia activation and protects mDA neurons from IFNγ-driven neurotoxicity. J Neurochem. 2015; 134(1):125-34. DOI: 10.1111/jnc.13111. View

17.

Shrestha B, Coykendall K, Li Y, Moon A, Priyadarshani P, Yao L . Repair of injured spinal cord using biomaterial scaffolds and stem cells. Stem Cell Res Ther. 2014; 5(4):91. PMC: 4282172. DOI: 10.1186/scrt480. View

18.

Oh S, Jeon S . Current Concept of Stem Cell Therapy for Spinal Cord Injury: A Review. Korean J Neurotrauma. 2016; 12(2):40-46. PMC: 5110917. DOI: 10.13004/kjnt.2016.12.2.40. View

19.

Assinck P, Duncan G, Hilton B, Plemel J, Tetzlaff W . Cell transplantation therapy for spinal cord injury. Nat Neurosci. 2017; 20(5):637-647. DOI: 10.1038/nn.4541. View