Skin Regeneration is Accelerated by a Lower Dose of Multipotent Mesenchymal Stromal/stem Cells-a Paradigm Change

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2021 Jan 26

PMID 33494813

Citations 13

Authors

Gertraud Eylert

Reinhard Dolp

Alexandra Parousis

Richard Cheng

Christopher Auger

Magdalena Holter

Ingrid Lang-Olip

Viola Reiner

Lars-Peter Kamolz

Marc G Jeschke

Affiliations

Soon will be listed here.

Abstract

Background: Multipotent mesenchymal stromal/stem cell (MSC) therapy is under investigation in promising (pre-)clinical trials for wound healing, which is crucial for survival; however, the optimal cell dosage remains unknown. The aim was to investigate the efficacy of different low-to-high MSC dosages incorporated in a biodegradable collagen-based dermal regeneration template (DRT) Integra®.

Methods: We conducted a porcine study (N = 8 Yorkshire pigs) and seeded between 200 and 2,000,000 cells/cm of umbilical cord mesenchymal stromal/stem cells on the DRT and grafted it onto full-thickness burn excised wounds. On day 28, comparisons were made between the different low-to-high cell dose groups, the acellular control, a burn wound, and healthy skin.

Result: We found that the low dose range between 200 and 40,000 cells/cm regenerates the full-thickness burn excised wounds most efficaciously, followed by the middle dose range of 200,000-400,000 cells/cm and a high dose of 2,000,000 cells/cm. The low dose of 40,000 cells/cm accelerated reepithelialization, reduced scarring, regenerated epidermal thickness superiorly, enhanced neovascularization, reduced fibrosis, and reduced type 1 and type 2 macrophages compared to other cell dosages and the acellular control.

Conclusion: This regenerative cell therapy study using MSCs shows efficacy toward a low dose, which changes the paradigm that more cells lead to better wound healing outcome.

Citing Articles

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RGDSP-functionalized peptide hydrogel stimulates growth factor secretion via integrin αv/PI3K/AKT axis for improved wound healing by human amniotic mesenchymal stem cells.

Wei W, Huang L, Chen L, He H, Liu Y, Feng Y Front Bioeng Biotechnol. 2024; 12:1385931.

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Younesi F, Hinz B Int J Mol Sci. 2024; 25(16).

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Dermal Regeneration Template in the Management and Reconstruction of Burn Injuries and Complex Wounds: A Review.

Gupta S, Moiemen N, Fischer J, Attinger C, Jeschke M, Taupin P Plast Reconstr Surg Glob Open. 2024; 12(3):e5674.

PMID: 38510326 PMC: 10954069. DOI: 10.1097/GOX.0000000000005674.

Allogenic adipose derived mesenchymal stem cells are effective than antibiotics in treating endometritis.

Bhaskar V, Saini S, Ansari S, Ghai S, Thakur A, Chopra S Sci Rep. 2023; 13(1):11280.

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