Effects of Antimicrobial Photodynamic Therapy on Organic Solution and Root Surface In Vitro

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2021 Jan 26

PMID 33494221

Citations 1

Authors

Yuji Matsushima

Akihiro Yashima

Meri Fukaya

Satoshi Shirakawa

Tomoko Ohshima

Tomomi Kawai

Takatoshi Nagano

Kazuhiro Gomi

Affiliations

Soon will be listed here.

Abstract

Antimicrobial photodynamic therapy (a-PDT) is attracting attention as a new form of dental treatment. While it is primarily applied to produce an antibacterial effect, it decreases lipopolysaccharide (LPS) and protease activity. Here, we evaluated differences in the antibacterial activity of a-PDT on three types of bacteria and the effects on the organic substances (i.e., albumin and LPS). Furthermore, we investigated the effects of a-PDT on root surfaces. A FotoSan630 and toluidine blue were used to perform a-PDT in this study. We measured its antimicrobial activity against , , and . Antimicrobial testing revealed strong antimicrobial action and , , and were almost undetectable after 50, 120, and 100 s, respectively. In organic resolution tests, albumin was significantly decreased from 1 min after a-PDT application onward, while LPS significantly decreased at 5 min after the application. The root surfaces after a-PDT were confirmed to be cleaner than the controls without suffering any damage. Depending on the bacterial species, a-PDT exhibited antimicrobial activity against various types of bacteria and sensitivity differed. Moreover, we reported that a-PDT resolves protein and LPS, enabling the formation of a healthy root surface without any damage.

Citing Articles

Antimicrobial photodynamic therapy against oral biofilm: influencing factors, mechanisms, and combined actions with other strategies.

Li Y, Sun G, Xie J, Xiao S, Lin C Front Microbiol. 2023; 14:1192955.

PMID: 37362926 PMC: 10288113. DOI: 10.3389/fmicb.2023.1192955.

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