Suppression of the NTS-CPS1 Regulatory Axis by AFF1 in Lung Adenocarcinoma Cells

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2021 Jan 25

PMID 33493519

Citations 1

Authors

Junjie Yue

Qian Dai

Shaohua Hao

ShiQi Zhu

Xiaoxu Liu

Zhiqun Tang

Meng Li

Haitong Fang

Chengqi Lin

Zhuojuan Luo

Affiliations

Soon will be listed here.

Abstract

Upregulation of the neuropeptide neurotensin (NTS) in a subgroup of lung cancers has been linked to poor prognosis. However, the regulatory pathway centered on NTS in lung cancer remains unclear. Here we identified the NTS-specific enhancer in lung adenocarcinoma cells. The AF4/FMR2 (AFF) family protein AFF1 occupies the NTS enhancer and inhibits NTS transcription. Clustering analysis of lung adenocarcinoma gene expression data demonstrated that NTS expression is highly positively correlated with the expression of the oncogenic factor CPS1. Detailed analyses demonstrated that the IL6 pathway antagonizes NTS in regulating CPS1. Thus, our analyses revealed a novel NTS-centered regulatory axis, consisting of AFF1 as a master transcription suppressor and IL6 as an antagonist in lung adenocarcinoma cells.

Citing Articles

Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation.

Che Z, Liu X, Dai Q, Fang K, Guo C, Yue J J Mol Cell Biol. 2023; 15(8).

PMID: 37528066 PMC: 11113081. DOI: 10.1093/jmcb/mjad049.

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