Protocol for Determining Zinc-dependent β Cell-selective Small-molecule Delivery in Mouse Pancreas

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2021 Jan 25

PMID 33490979

Authors

Timothy M Horton

Benjamin R Kraemer

Justin P Annes

Affiliations

Soon will be listed here.

Abstract

Targeted drug delivery to pancreatic islet β cells is an unmet clinical need. β cells possess a uniquely high Zn concentration, and integrating Zn-binding activity into a small molecule can bias drug accumulation and activity toward β cells. This protocol can be used to evaluate a molecule's capacity to chelate islet Zn, accumulate in islets, and stimulate β cell-selective replication in mouse pancreas. One obstacle is establishing an LC-MS/MS-based method for compound measurement. Limitations include target compound ionizability and the time-sensitive nature of some experimental assay steps. For complete details on the use and execution of this protocol, please refer to Horton et al. (2019).

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