Adipose-Derived Stem Cells: Current Applications and Future Directions in the Regeneration of Multiple Tissues

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2021 Jan 25

PMID 33488736

Citations 77

Authors

Jiaxin Zhang

Yuzhe Liu

Yutong Chen

Lei Yuan

He Liu

Jincheng Wang

Qiran Liu

Yan Zhang

Affiliations

Soon will be listed here.

Abstract

Adipose-derived stem cells (ADSCs) can maintain self-renewal and enhanced multidifferentiation potential through the release of a variety of paracrine factors and extracellular vesicles, allowing them to repair damaged organs and tissues. Consequently, considerable attention has increasingly been paid to their application in tissue engineering and organ regeneration. Here, we provide a comprehensive overview of the current status of ADSC preparation, including harvesting, isolation, and identification. The advances in preclinical and clinical evidence-based ADSC therapy for bone, cartilage, myocardium, liver, and nervous system regeneration as well as skin wound healing are also summarized. Notably, the perspectives, potential challenges, and future directions for ADSC-related researches are discussed. We hope that this review can provide comprehensive and standardized guidelines for the safe and effective application of ADSCs to achieve predictable and desired therapeutic effects.

Citing Articles

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References

Muangsanit P, Shipley R, Phillips J . Vascularization Strategies for Peripheral Nerve Tissue Engineering. Anat Rec (Hoboken). 2018; 301(10):1657-1667. PMC: 6282999. DOI: 10.1002/ar.23919. View

Zomer H, da Silva Jeremias T, Ratner B, Trentin A . Mesenchymal stromal cells from dermal and adipose tissues induce macrophage polarization to a pro-repair phenotype and improve skin wound healing. Cytotherapy. 2020; 22(5):247-260. DOI: 10.1016/j.jcyt.2020.02.003. View

Varghese J, Griffin M, Mosahebi A, Butler P . Systematic review of patient factors affecting adipose stem cell viability and function: implications for regenerative therapy. Stem Cell Res Ther. 2017; 8(1):45. PMC: 5329955. DOI: 10.1186/s13287-017-0483-8. View

Salazar-Noratto G, Luo G, Denoeud C, Padrona M, Moya A, Bensidhoum M . Understanding and leveraging cell metabolism to enhance mesenchymal stem cell transplantation survival in tissue engineering and regenerative medicine applications. Stem Cells. 2019; 38(1):22-33. DOI: 10.1002/stem.3079. View

Liang H, Ding X, Yu Y, Zhang H, Wang L, Kan Q . Adipose-derived mesenchymal stem cells ameliorate acute liver injury in rat model of CLP induced-sepsis via sTNFR1. Exp Cell Res. 2019; 383(1):111465. DOI: 10.1016/j.yexcr.2019.06.010. View

Deng Z, Li Y, Gao X, Lei G, Huard J . Bone morphogenetic proteins for articular cartilage regeneration. Osteoarthritis Cartilage. 2018; 26(9):1153-1161. DOI: 10.1016/j.joca.2018.03.007. View

Urrutia D, Caviedes P, Mardones R, Minguell J, Vega-Letter A, Jofre C . Comparative study of the neural differentiation capacity of mesenchymal stromal cells from different tissue sources: An approach for their use in neural regeneration therapies. PLoS One. 2019; 14(3):e0213032. PMC: 6437714. DOI: 10.1371/journal.pone.0213032. View

Thesleff T, Lehtimaki K, Niskakangas T, Huovinen S, Mannerstrom B, Miettinen S . Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results. Stem Cells Transl Med. 2017; 6(7):1576-1582. PMC: 5689754. DOI: 10.1002/sctm.16-0410. View

Yan Y, Fang J, Wen X, Teng X, Li B, Zhou Z . Therapeutic applications of adipose-derived mesenchymal stem cells on acute liver injury in canines. Res Vet Sci. 2019; 126:233-239. DOI: 10.1016/j.rvsc.2019.09.004. View

10.

Tong S, Yin J, Liu J . Platelet-rich plasma has beneficial effects in mice with osteonecrosis of the femoral head by promoting angiogenesis. Exp Ther Med. 2018; 15(2):1781-1788. PMC: 5776555. DOI: 10.3892/etm.2017.5655. View

11.

Wong C, Xu Y, Liu X, Xu S, Zhang Y, Zhu Z . Effect of Induction Time on the Proliferation and Differentiation of Induced Schwann-Like Cells from Adipose-Derived Stem Cells. Cell Mol Neurobiol. 2020; 40(7):1105-1116. PMC: 11448847. DOI: 10.1007/s10571-020-00795-5. View

12.

Sterodimas A, de Faria J, Nicaretta B, Pitanguy I . Tissue engineering with adipose-derived stem cells (ADSCs): current and future applications. J Plast Reconstr Aesthet Surg. 2009; 63(11):1886-92. DOI: 10.1016/j.bjps.2009.10.028. View

13.

Bian Y, Deng C, Li W, Lei Z, Li Y, Li X . A Comparative Study on the Biological Characteristics of Human Adipose-Derived Stem Cells from Lipectomy and Liposuction. PLoS One. 2016; 11(9):e0162343. PMC: 5017729. DOI: 10.1371/journal.pone.0162343. View

14.

Chae Y, Won Jun D, Lee J, Saeed W, Kang H, Jang K . The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury. Gut Liver. 2019; 13(4):450-460. PMC: 6622567. DOI: 10.5009/gnl18235. View

15.

Barlian A, Judawisastra H, Alfarafisa N, Wibowo U, Rosadi I . Chondrogenic differentiation of adipose-derived mesenchymal stem cells induced by L-ascorbic acid and platelet rich plasma on silk fibroin scaffold. PeerJ. 2018; 6:e5809. PMC: 6250097. DOI: 10.7717/peerj.5809. View

16.

Markarian C, Frey G, Silveira M, Chem E, Milani A, Ely P . Isolation of adipose-derived stem cells: a comparison among different methods. Biotechnol Lett. 2013; 36(4):693-702. DOI: 10.1007/s10529-013-1425-x. View

17.

Siclari A, Mascaro G, Gentili C, Kaps C, Cancedda R, Boux E . Cartilage repair in the knee with subchondral drilling augmented with a platelet-rich plasma-immersed polymer-based implant. Knee Surg Sports Traumatol Arthrosc. 2013; 22(6):1225-34. DOI: 10.1007/s00167-013-2484-1. View

18.

Ardeshirylajimi A, Delgoshaie M, Mirzaei S, Khojasteh A . Different Porosities of Chitosan Can Influence the Osteogenic Differentiation Potential of Stem Cells. J Cell Biochem. 2017; 119(1):625-633. DOI: 10.1002/jcb.26223. View

19.

Raposio E, Caruana G, Bonomini S, Libondi G . A novel and effective strategy for the isolation of adipose-derived stem cells: minimally manipulated adipose-derived stem cells for more rapid and safe stem cell therapy. Plast Reconstr Surg. 2014; 133(6):1406-1409. DOI: 10.1097/PRS.0000000000000170. View

20.

Bhartiya D . Clinical Translation of Stem Cells for Regenerative Medicine. Circ Res. 2019; 124(6):840-842. DOI: 10.1161/CIRCRESAHA.118.313823. View