MiR-335-5p Suppresses Gastric Cancer Progression by Targeting MAPK10

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2021 Jan 23

PMID 33482821

Citations 14

Authors

Yi Gao

Yanfeng Wang

Xiaofei Wang

Changan Zhao

Fenghui Wang

Juan Du

Huahua Zhang

Haiyan Shi

Yun Feng

Dan Li

Jing Yan

Yan Yao

Weihong Hu

Ruxin Ding

Mengjie Zhang

Lumin Wang

Chen Huang

Jing Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated.

Methods: Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting.

Results: The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p.

Conclusions: These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression.

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