The Effect of Synthetic Bone Graft Substitutes on Bone Formation in Rabbit Calvarial Defects

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2021 Jan 21

PMID 33475862

Citations 4

Authors

Nikola Saulacic

Masako Fujioka-Kobayashi

Yasushi Kimura

Ava Insa Bracher

Claudio Zihlmann

Niklaus P Lang

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to evaluate the influence of the intensity of the biomimetic hydroxyapatite (HA) coating of α-tricalcium phosphate (α-TCP) on biomaterial degradation and bone formation. Twenty-four female NZW rabbits of approximately 12 weeks of age were used. Critical size defects were randomly treated with 3%:97% HA:α-TCP (BBCP1), 12%:88% HA:α-TCP (BBCP2), and 23%:77% HA:α-TCP (BBCP3), respectively or sham. All defects were covered with a resorbable collagen membrane. Animals were euthanized after 3 and 12 weeks of healing and samples were investigated by micro-CT and histologic analysis. Ingrowth of newly formed woven bone from the original bone at 3-week healing period was observed in all samples. At the 12-week healing period, the new bone in the peripheral area was mainly lamellar and in the central region composed of both woven and lamellar bone. New bony tissue was found on the surface of all three types of granules and at the interior of the BBCP1 granules. Samples with 3% HA showed significantly less residual biomaterial in comparison to the other two groups. Furthermore, BBCP1 significantly promoted new bone area as compared to other three groups and more bone volume as compared to the control. Within its limitations, this study indicated the highest degradation rate in case of BBCP1 concomitant with the highest rate of bone formation. Hence, formation of new bone can be affected by the level of biomimetic HA coating of α-TCP.

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References

Mordenfeld A, Hallman M, Johansson C, Albrektsson T . Histological and histomorphometrical analyses of biopsies harvested 11 years after maxillary sinus floor augmentation with deproteinized bovine and autogenous bone. Clin Oral Implants Res. 2010; 21(9):961-70. DOI: 10.1111/j.1600-0501.2010.01939.x. View

Wang H, Zhi W, Lu X, Li X, Duan K, Duan R . Comparative studies on ectopic bone formation in porous hydroxyapatite scaffolds with complementary pore structures. Acta Biomater. 2013; 9(9):8413-21. DOI: 10.1016/j.actbio.2013.05.026. View

Wang R, Lang N . Ridge preservation after tooth extraction. Clin Oral Implants Res. 2012; 23 Suppl 6:147-56. DOI: 10.1111/j.1600-0501.2012.02560.x. View

Park J, Kim E, Jang J, Suh J, Park K, Hanawa T . Healing of rabbit calvarial bone defects using biphasic calcium phosphate ceramics made of submicron-sized grains with a hierarchical pore structure. Clin Oral Implants Res. 2010; 21(3):268-76. DOI: 10.1111/j.1600-0501.2009.01846.x. View

Rojbani H, Nyan M, Ohya K, Kasugai S . Evaluation of the osteoconductivity of α-tricalcium phosphate, β-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. J Biomed Mater Res A. 2011; 98(4):488-98. DOI: 10.1002/jbm.a.33117. View

Sawada K, Nakahara K, Haga-Tsujimura M, Iizuka T, Fujioka-Kobayashi M, Igarashi K . Comparison of three block bone substitutes for bone regeneration: long-term observation in the beagle dog. Odontology. 2018; 106(4):398-407. DOI: 10.1007/s10266-018-0352-7. View

Carrodeguas R, De Aza S . α-Tricalcium phosphate: synthesis, properties and biomedical applications. Acta Biomater. 2011; 7(10):3536-46. DOI: 10.1016/j.actbio.2011.06.019. View

Eliaz N, Metoki N . Calcium Phosphate Bioceramics: A Review of Their History, Structure, Properties, Coating Technologies and Biomedical Applications. Materials (Basel). 2017; 10(4). PMC: 5506916. DOI: 10.3390/ma10040334. View

Carrel J, Wiskott A, Scherrer S, Durual S . Large Bone Vertical Augmentation Using a Three-Dimensional Printed TCP/HA Bone Graft: A Pilot Study in Dog Mandible. Clin Implant Dent Relat Res. 2016; 18(6):1183-1192. DOI: 10.1111/cid.12394. View

10.

Titsinides S, Agrogiannis G, Karatzas T . Bone grafting materials in dentoalveolar reconstruction: A comprehensive review. Jpn Dent Sci Rev. 2019; 55(1):26-32. PMC: 6354279. DOI: 10.1016/j.jdsr.2018.09.003. View

11.

Wang L, Barbieri D, Zhou H, de Bruijn J, Bao C, Yuan H . Effect of particle size on osteoinductive potential of microstructured biphasic calcium phosphate ceramic. J Biomed Mater Res A. 2014; 103(6):1919-29. DOI: 10.1002/jbm.a.35325. View

12.

Jensen S, Bornstein M, Dard M, Bosshardt D, Buser D . Comparative study of biphasic calcium phosphates with different HA/TCP ratios in mandibular bone defects. A long-term histomorphometric study in minipigs. J Biomed Mater Res B Appl Biomater. 2008; 90(1):171-81. DOI: 10.1002/jbm.b.31271. View

13.

Habibovic P, Yuan H, van der Valk C, Meijer G, van Blitterswijk C, de Groot K . 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials. 2004; 26(17):3565-75. DOI: 10.1016/j.biomaterials.2004.09.056. View

14.

Daculsi G, Laboux O, Malard O, Weiss P . Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med. 2004; 14(3):195-200. DOI: 10.1023/a:1022842404495. View

15.

Helder M, van Esterik F, Kwehandjaja M, Ten Bruggenkate C, Klein-Nulend J, Schulten E . Evaluation of a new biphasic calcium phosphate for maxillary sinus floor elevation: Micro-CT and histomorphometrical analyses. Clin Oral Implants Res. 2018; 29(5):488-498. PMC: 6001541. DOI: 10.1111/clr.13146. View

16.

Nasu T, Takemoto M, Akiyama N, Fujibayashi S, Neo M, Nakamura T . EP4 agonist accelerates osteoinduction and degradation of beta-tricalcium phosphate by stimulating osteoclastogenesis. J Biomed Mater Res A. 2008; 89(3):601-8. DOI: 10.1002/jbm.a.31984. View

17.

Nkenke E, Stelzle F . Clinical outcomes of sinus floor augmentation for implant placement using autogenous bone or bone substitutes: a systematic review. Clin Oral Implants Res. 2009; 20 Suppl 4:124-33. DOI: 10.1111/j.1600-0501.2009.01776.x. View

18.

Hwang J, Park J, Lee J, Jung U, Kim C, Cho K . Comparative evaluation of three calcium phosphate synthetic block bone graft materials for bone regeneration in rabbit calvaria. J Biomed Mater Res B Appl Biomater. 2012; 100(8):2044-52. DOI: 10.1002/jbm.b.32768. View

19.

Kolerman R, Samorodnitzky-Naveh G, Barnea E, Tal H . Histomorphometric analysis of newly formed bone after bilateral maxillary sinus augmentation using two different osteoconductive materials and internal collagen membrane. Int J Periodontics Restorative Dent. 2012; 32(1):e21-8. View

20.

Davison N, Ten Harkel B, Schoenmaker T, Luo X, Yuan H, Everts V . Osteoclast resorption of beta-tricalcium phosphate controlled by surface architecture. Biomaterials. 2014; 35(26):7441-51. DOI: 10.1016/j.biomaterials.2014.05.048. View