Cell Therapy: A Potential Solution for the Healing of Bone Cavities

Overview

Journal Heliyon

Specialty Social Sciences

Date 2021 Jan 21

PMID 33474509

Authors

Sara El-Gindy

Maram Farouk Obeid

Kareim Mostafa Elbatouty

Elham Elshaboury

Ehab Hassanien

Affiliations

Soon will be listed here.

Abstract

Aim: To Explore whether the use of autologous BMMNCs as a cell therapy technique will improve the healing of bone cavities in vivo.

Methodology: After achieving proper anesthesia, mononuclear cells were isolated from iliac crest's bone marrow aspirates (BMMNCs). Then access cavity, root canal preparation, and filling were done in third and fourth premolars, followed by amalgam coronal restoration. After that, a flap was reflected and a standardized bone cavity was drilled, the related root-ends were resected and retrocavity was drilled and filled with MTA. Before repositioning the flap, the bone cavity was filled with the desired filling material according to its corresponding group (n = 8): CollaCote group; where collagen scaffold was used, MNC group; in which CollaCote® loaded with isolated BMMNCs were applied, Biogen group; in which BIO-GEN® graft material was applied and finally Control group; where the bone cavities were left empty to heal spontaneously. Evaluations of healing of the bone cavities were done radiographically and histologically.

Results: The MNC group induced the best healing potential with statistical significant difference from other groups.

Conclusion: cell therapy utilizing autologous BMMNCs looks to beat the conventional therapies and convey a significant improvement in the healing of the bone cavity in vivo.

References

FRIEDENSTEIN A, PETRAKOVA K, Kurolesova A, Frolova G . Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968; 6(2):230-47. View

Verboket R, Leiblein M, Seebach C, Nau C, Janko M, Bellen M . Autologous cell-based therapy for treatment of large bone defects: from bench to bedside. Eur J Trauma Emerg Surg. 2018; 44(5):649-665. PMC: 6182650. DOI: 10.1007/s00068-018-0906-y. View

Arinzeh T, Tran T, Mcalary J, Daculsi G . A comparative study of biphasic calcium phosphate ceramics for human mesenchymal stem-cell-induced bone formation. Biomaterials. 2004; 26(17):3631-8. DOI: 10.1016/j.biomaterials.2004.09.035. View

Jager M, Hernigou P, Zilkens C, Herten M, Li X, Fischer J . Cell therapy in bone healing disorders. Orthop Rev (Pavia). 2011; 2(2):e20. PMC: 3143975. DOI: 10.4081/or.2010.e20. View

Fernandez-Bances I, Perez-Basterrechea M, Perez-Lopez S, Nunez Batalla D, Fernandez Rodriguez M, Alvarez-Viejo M . Repair of long-bone pseudoarthrosis with autologous bone marrow mononuclear cells combined with allogenic bone graft. Cytotherapy. 2013; 15(5):571-7. DOI: 10.1016/j.jcyt.2013.01.004. View

Chandler N, Koshy S . The changing role of the apicectomy operation in dentistry. J R Coll Surg Edinb. 2002; 47(5):660-7. View

Young C, Terada S, Vacanti J, Honda M, Bartlett J, Yelick P . Tissue engineering of complex tooth structures on biodegradable polymer scaffolds. J Dent Res. 2002; 81(10):695-700. DOI: 10.1177/154405910208101008. View

Ratajczak M, Kucia M, Majka M, Reca R, Ratajczak J . Heterogeneous populations of bone marrow stem cells--are we spotting on the same cells from the different angles?. Folia Histochem Cytobiol. 2004; 42(3):139-46. View

de Paula-Silva F, Hassan B, da Silva L, Leonardo M, Wu M . Outcome of root canal treatment in dogs determined by periapical radiography and cone-beam computed tomography scans. J Endod. 2009; 35(5):723-6. DOI: 10.1016/j.joen.2009.01.023. View

10.

Kruse C, Spin-Neto R, Christiansen R, Wenzel A, Kirkevang L . Periapical Bone Healing after Apicectomy with and without Retrograde Root Filling with Mineral Trioxide Aggregate: A 6-year Follow-up of a Randomized Controlled Trial. J Endod. 2016; 42(4):533-7. DOI: 10.1016/j.joen.2016.01.011. View

11.

Cancedda R, Mastrogiacomo M, Bianchi G, Derubeis A, Muraglia A, Quarto R . Bone marrow stromal cells and their use in regenerating bone. Novartis Found Symp. 2003; 249:133-43; discussion 143-7, 170-4, 239-41. View

12.

Tawfik H, Abu-Seida A, Hashem A, Nagy M . Regenerative potential following revascularization of immature permanent teeth with necrotic pulps. Int Endod J. 2013; 46(10):910-22. DOI: 10.1111/iej.12079. View

13.

Wang H, Al-Shammari K . HVC ridge deficiency classification: a therapeutically oriented classification. Int J Periodontics Restorative Dent. 2002; 22(4):335-43. View

14.

Obeid M, Saber S, Ismael A, Hassanien E . Mesenchymal stem cells promote hard-tissue repair after direct pulp capping. J Endod. 2013; 39(5):626-31. DOI: 10.1016/j.joen.2012.12.012. View

15.

Lui J, Khin M, Krishnaswamy G, Chen N . Prognostic factors relating to the outcome of endodontic microsurgery. J Endod. 2014; 40(8):1071-6. DOI: 10.1016/j.joen.2014.04.005. View

16.

Wlodarski K, Galus R, Wlodarski P . Non-adherent bone marrow cells are a rich source of cells forming bone in vivo. Folia Biol (Praha). 2004; 50(5):167-73. View

17.

Jager M, Jelinek E, Wess K, Scharfstadt A, Jacobson M, Kevy S . Bone marrow concentrate: a novel strategy for bone defect treatment. Curr Stem Cell Res Ther. 2009; 4(1):34-43. DOI: 10.2174/157488809787169039. View

18.

Henkel K, Gerber T, Dorfling P, Gundlach K, Bienengraber V . Repair of bone defects by applying biomatrices with and without autologous osteoblasts. J Craniomaxillofac Surg. 2005; 33(1):45-9. DOI: 10.1016/j.jcms.2004.08.005. View

19.

Sen R, Tripathy S, Aggarwal S, Marwaha N, Sharma R, Khandelwal N . Early results of core decompression and autologous bone marrow mononuclear cells instillation in femoral head osteonecrosis: a randomized control study. J Arthroplasty. 2011; 27(5):679-86. DOI: 10.1016/j.arth.2011.08.008. View

20.

Suneelkumar C, Datta K, Srinivasan M, Kumar S . Biphasic calcium phosphate in periapical surgery. J Conserv Dent. 2010; 11(2):92-6. PMC: 2813096. DOI: 10.4103/0972-0707.44059. View