» Articles » PMID: 33473432

Complete Mitochondrial Genome of a DHA-rich Protist Sp. TIO1101

Overview
Date 2021 Jan 21
PMID 33473432
Citations 2
Authors
Affiliations
Soon will be listed here.
Abstract

sp. TIO1101 is a crucial commercial alga used to produce docosahexaenoic acid (DHA), a long-chain polyunsaturated fatty acid that is beneficial for human health. In this study, we sequenced the mitochondrial genome (mitogenome) of sp. TIO1101 for the first time using an Illumina HiSeq 2500 system (Illumina Inc., San Deigo, CA). The assembled mitogenome was 31 494 bp long with 33.92% GC content. The mitogenome contains 56 genes, including 33 protein-coding genes, 21 transfer RNA genes and two ribosomal RNA genes. Maximum-likelihood phylogenetic analysis of sp. TIO1101 showed that it was most closely related to among the examined species.

Citing Articles

Low-temperature effects on docosahexaenoic acid biosynthesis in sp. TIO01 and its proposed underlying mechanism.

Hu F, Clevenger A, Zheng P, Huang Q, Wang Z Biotechnol Biofuels. 2020; 13:172.

PMID: 33088342 PMC: 7565746. DOI: 10.1186/s13068-020-01811-y.


Transcriptomic Analysis of the Regulation of Lipid Fraction Migration and Fatty Acid Biosynthesis in Schizochytrium sp.

Ren L, Hu X, Zhao X, Chen S, Wu Y, Li D Sci Rep. 2017; 7(1):3562.

PMID: 28620184 PMC: 5472558. DOI: 10.1038/s41598-017-03382-9.

References
1.
Metz J, Roessler P, Facciotti D, Levering C, Dittrich F, Lassner M . Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science. 2001; 293(5528):290-3. DOI: 10.1126/science.1059593. View

2.
Fan K, Jiang Y, Faan Y, Chen F . Lipid characterization of mangrove thraustochytrid--Schizochytrium mangrovei. J Agric Food Chem. 2007; 55(8):2906-10. DOI: 10.1021/jf070058y. View

3.
Cheng R, Ge Y, Yang B, Zhong X, Lin X, Huang Z . Cloning and functional analysis of putative malonyl-CoA:acyl-carrier protein transacylase gene from the docosahexaenoic acid-producer Schizochytrium sp. TIO1101. World J Microbiol Biotechnol. 2013; 29(6):959-67. DOI: 10.1007/s11274-013-1253-0. View

4.
Wyman S, Jansen R, Boore J . Automatic annotation of organellar genomes with DOGMA. Bioinformatics. 2004; 20(17):3252-5. DOI: 10.1093/bioinformatics/bth352. View

5.
Stamatakis A . RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006; 22(21):2688-90. DOI: 10.1093/bioinformatics/btl446. View